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11244304930
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note
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The final yield of homogeneous GS4 was further reduced because GS4 and GS4* could only be partially resolved on preparative scale HPLC, even using optimized gradients and elevated temperatures.
-
-
-
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12
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11244316875
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In contrast with the present model study, Backes and Ellman did not observe significant S-alkylation in a Met-containing dipeptide (ref 7b)
-
In contrast with the present model study, Backes and Ellman did not observe significant S-alkylation in a Met-containing dipeptide (ref 7b).
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-
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13
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11244255970
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4
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4
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14
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11244281525
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note
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2.
-
-
-
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15
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11244327107
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Addition of 350 mM 1,6-hexanediol was found to reduce nonspecific aggregation to 25% of total protein, as compared to 60% in its absence
-
Addition of 350 mM 1,6-hexanediol was found to reduce nonspecific aggregation to 25% of total protein, as compared to 60% in its absence.
-
-
-
-
16
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11244265352
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note
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In a typical ligation reaction, 3 μmol of GS4-Nle (7.5 mg) was mixed with 218 nmol of TβR-1Δ (7.5 mg) in 4.5 mL of 100 mM Hepes pH 8.0, 100 mM okadaic acid, 0.5 mM NaCl, 350 mM 1,6 hexanediol, and 50 mM MESNA at 4 °C for 16 h.
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-
-
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17
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11244315773
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note
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32P]ATP. The level of Smad phosphorylation was analyzed by SDS-PAGE and autoradiography.
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18
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0028842064
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