Identification of in vivo essential genes from Pseudomonas aeruginosa by PCR-based signature-tagged mutagenesis

FEMS Microbiology Letters

Volumn 210, Issue 1, 2002, Pages 73-80

  Lehoux, Dario E ^a,b   Sanschagrin, François ^a   Levesque, Roger C ^a  

^a UNIVERSITÉ LAVAL   (Canada)

^b Centre de Recherche   (Canada)

Author keywords

In vivo; Pseudomonas aeruginosa; Signature tagged mutagenesis; Virulence

Indexed keywords

ABC TRANSPORTER; BACTERIAL PROTEIN;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM MUTANT; CATION TRANSPORT; CONTROLLED STUDY; DATA BASE; FEMALE; GENE INSERTION; IN VIVO STUDY; LUNG INFECTION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PSEUDOMONAS AERUGINOSA; RAT; SEQUENCE HOMOLOGY; STRESS; TRANSPOSON;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; GENES, ESSENTIAL; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; TRANSPOSONS;

EID: 0037161213     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00597-9     Document Type: Article

References (29)

1. Strategies for isolation of in vivo expressed genes from bacteria
2. Discovering essential and infection-related genes
3. Simultaneous identification of bacterial virulence genes by negative selection
4. Whole genome scan for habitat-specific genes by signature-tagged mutagenesis
5. Detection of genes essential in specific niches by signature-tagged mutagenesis
6. Defined oligonucleotide tag pools and PCR screening in signature-tagged mutagenesis of essential genes from bacteria
7. A rat model of chronic respiratory infection with Pseudomonas aeruginosa
8. A functional genomic analysis of type 3 Streptococcus pneumoniae virulence
9. Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells
10. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen
11. Signature-tagged and directed mutagenesis identify PABA synthetase as essential for Aspergillus fumigatus pathogenicity
12. Selection of bacterial virulence genes that are specifically induced in host tissues
13. Genomics of the 35-kb pvd locus and analysis of novel pvdIJK genes implicated in pyoverdine biosynthesis in Pseudomonas aeruginosa
14. Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection
15. Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes
16. Characterization of genes involved in molybdenum transport in Azotobacter vinelandii
17. Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli
18. Helicobacter pylori uptake and efflux: Basis for intrinsic susceptibility to antibiotics in vitro
19. The acrAB homolog of Haemophilus influenzae codes for a functional multidrug efflux pump
20. Resistance of Neisseria gonorrhoeae to antimicrobial hydrophobic agents is modulated by the mtrRCDE efflux system
21. Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa
22. Resistance mechanisms in Pseudomonas aeruginosa and other nonfermentative gram-negative bacteria
23. Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones
24. Roles of the Escherichia coli small heat shock proteins IbpA and IbpB in thermal stress management: Comparison with ClpA, ClpB, and HtpG in vivo
25. Identification and characterization of in vivo attenuated mutants of Brucella melitensis
26. Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa