Inducible expression of Bcl-XL restricts apoptosis resistance to the antibody secretion phase in hybridoma cultures

Biotechnology and Bioengineering

Volumn 79, Issue 2, 2002, Pages 180-187

  Jung, Daniel ^a,b   Côté, Serge ^a,b   Drouin, Mathieu ^a   Simard, Carl ^a   Lemieux, Réal ^a  

^a HÉMA QUÉBEC   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

Apoptosis; Bcl XL; Hybridoma; Inducible expression

Indexed keywords

ANTIBODIES; CELL CULTURE; GENES;

GENETIC EFFECTS;

BIOTECHNOLOGY;

METALLOTHIONEIN; PROTEIN BCL XL; BCL2L1 PROTEIN, HUMAN; BCL2L1 PROTEIN, MOUSE; MONOCLONAL ANTIBODY; NOCODAZOLE; PROTEIN BCL 2; PROTEIN BCL X; ZINC SULFATE;

ANIMAL CELL; ANTIBODY PRODUCTION; APOPTOSIS; ARTICLE; CELL CYCLE; CELL DENSITY; CELL VIABILITY; CONTROLLED STUDY; EXPRESSION VECTOR; GENE INDUCTION; GENETIC TRANSFECTION; HYBRIDOMA CELL CULTURE; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; ANIMAL; BIOLOGICAL MODEL; BIOSYNTHESIS; CELL LINE; CELL SURVIVAL; COMPARATIVE STUDY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; HUMAN; HYBRIDOMA; IMMUNOLOGY; MOUSE; PHYSIOLOGY; REFERENCE VALUE;

ANIMAL; ANTIBODIES, MONOCLONAL; APOPTOSIS; CELL LINE; CELL SURVIVAL; COMPARATIVE STUDY; GENE EXPRESSION REGULATION; HUMAN; HYBRIDOMAS; METALLOTHIONEIN; MICE; MODELS, GENETIC; NOCODAZOLE; PROMOTER REGIONS (GENETICS); PROTO-ONCOGENE PROTEINS C-BCL-2; REFERENCE VALUES; TRANSCRIPTION, GENETIC; TRANSFECTION; ZINC SULFATE; ANIMALS; BCL-X PROTEIN; HUMANS;

MAMMALIA; VECTORS;

EID: 0037142774     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10279     Document Type: Article

References (32)

1. Bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death
2. Prolongation of murine hybridoma cell survival in stationary batch culture by Bcl-xL expression
3. Suppression of apoptosis by Bcl-2 or Bcl-xL promotes susceptibility to mutagenesis
4. Long term perfusion culture of hybridoma: A grow or die cell cycle system
5. Comparison of various methods for monitoring hybridoma cell proliferation
6. Reduction of background problems in nonradioactive northern and southern blot enables higher sensitivity than 32P-based hybridations
7. Induction of apoptosis in fibroblasts by c-myc protein
8. Cloning of a bcl-2 homologue by interaction with adenovirus EIB 19K
9. Effect of Bcl-2 expression on hybridoma cell growth in serum-supplemented protein-free and diluted media
10. Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth
11. Role of bcl-X(L) in the control of apoptosis in murine myeloma cells
12. Use of intracellular pH and annexin-V flow cytometric assays to monitor apoptosis and its suppression by bcl-2 over-expression in hybridoma cell culture
13. Metallothionein gene regulation in mouse cells
14. Characterization of higher avidity monoclonal antibodies produced by murine B-cell hybridoma variants selected for increased antigen binding of membrane Ig
15. Understanding factors that limit the productivity of suspension-based perfusion cultures operated at high medium renewal rates
16. Filtration based perfusion of hybridoma cultures in protein free medium: Reduction of membrane fouling by medium supplementation with DNase I
17. Dose-dependent reduction of apoptosis in nutrient-limited cultures of NS/O myeloma cells transfected with the E1B-19K adenoviral gene
18. Induction of apoptosis in oxygen-deprived cultures of hybridoma cells
19. Expression of Bcl-xL and loss of p53 can cooperate to overcome a cell cycle checkpoint induced by mitotic spindle damage
20. Expression of bcl-xL can confer a multidrug resistance phenotype
21. NSO myeloma cell death: Influence of Bcl-2 overexpression
22. Bcl-2 heterodimerizes in vivo with a conserved homolog. Bax, that accelerates programmed cell death
23. Rapid apoptotic cell death of B-cell hybridomas in absence of gene expression
24. Essential role of optimal protein synthesis in preventing the apoptotic death of cultured B cell hybridomas
25. Bcl-2 and the regulation of programmed cell death
26. Monoclonal antibody productivity and the metabolic pattern of perfusion cultures under varying oxygen tension
27. Enhancement of survivability of mammalian cells by overexpression of the apoptosis-supressor gene Bcl-2
28. Regulation of apoptosis by viral gene products
29. Anti-apoptotic genes, bag-1 and bcl-2 enabled hybridoma cells to survive under treatment for arresting cell cycle
30. Kinetics of development of spontaneous apoptosis in B cell hybridoma cultures
31. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death
32. Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6