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Volumn 298, Issue 5593, 2002, Pages 572-576

Shadows cast by retinal blood vessels mapped in primary visual cortex

Author keywords

[No Author keywords available]

Indexed keywords

BIODIVERSITY; VISION;

EID: 0037131216     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1074887     Document Type: Article
Times cited : (56)

References (38)
  • 21
    • 2142771995 scopus 로고    scopus 로고
    • note
    • Experiments were performed on 12 adult squirrel monkeys from the California Regional Primate Center, Davis, CA. All procedures were approved by the University of California, San Francisco, Committee on Animal Research. Each animal was anesthetized with 2% isoflurane and paralyzed with succinylcholine (45 mg/kg, intravenously). The animal was placed in a stereotaxic frame mounted on a tripod to orient the visual axis perpendicular to a large tangent screen. Photographs were taken of both retinae and landmarks were projected onto a tangent screen with a fundus camera to pinpoint their eccentricity. Retinal magnification factor was 161 μm per degree in the central 24°. One eye was then enucleated under sterile conditions. Buprenorphine (0.03 mg/kg) was administered for 48 hours after surgery for analgesia. After a survival time of at least 10 days, the animal was killed with sodium pentobarbital (150 mg/kg) and perfused with 1% paraformaldehyde. Flatmounts were cut at 30 to 40 μm, air-dried on slides, reacted for CO, and montaged with Photoshop 6.0.
  • 25
    • 2142694645 scopus 로고    scopus 로고
    • note
    • 3H]proline were reconstituted in 20 μl of saline and injected into the vitreous of one eye. Alternate cortical sections were processed for CO or autoradiography.
  • 28
    • 2142827366 scopus 로고    scopus 로고
    • note
    • Entrance pupil size was measured by photography with a 100-mm macro-lens in five squirrel monkeys aged 1 week; it was 1.9 ± 0.03 mm (SEM), with a range of 1.7 to 2.0 mm. This value was used for exit pupil size, because for small pupils, the difference between entrance and exit pupil size is negligible. Distance from pupil to retina was measured in three newborn squirrel monkeys with Amode ultrasound; it was 8.4 ± 0.12 mm (SEM). Adult retinae were sectioned at 1 μm in Epon-Araldite and the lumen diameter of identified blood vessels was measured. Distance from retinal vessel to photoreceptors was also measured in these sections. Correction was made for retinal growth and tissue shrinkage.
  • 29
    • 2142820268 scopus 로고    scopus 로고
    • note
    • -1.85, where E is eccentricity.
  • 38
    • 2142700335 scopus 로고    scopus 로고
    • note
    • This work was supported by the National Eye Institute (grant R01 EY10217 and core grant EY02162), That Man May See, The Bunter Fund, and a Lew W. Wasserman Merit Award from Research to Prevent Blindness. We thank D. Hocking and I. Wood for help with tissue processing and R. Troyer for assistance with animal care and surgery. We thank the California Regional Primate Research Center, which is supported by NIH Base Grant RR00169. L. Sincich, N. Swindale, and G. Westheimer provided valuable comments on the manuscript.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.