Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells

Gene

Volumn 298, Issue 1, 2002, Pages 91-99

  Weisenberger, Daniel J ^a   Velicescu, Mihaela ^a   Preciado Lopez, Miguel A ^a   Gonzales, Felicidad A ^a   Tsai, Yvonne C ^a   Liang, Gangning ^a   Jones, Peter A ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

5 Untranslated region; Alternative splicing; DNA methylation; DNMT3a; Exon 1

Indexed keywords

DNA METHYLTRANSFERASE; ISOENZYME; MESSENGER RNA;

5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; BINDING SITE; CELL TYPE; CONTROLLED STUDY; CPG ISLAND; EMBRYO; EMBRYO CELL; EMBRYO DEVELOPMENT; EXON; GENE EXPRESSION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC VARIABILITY; HUMAN; HUMAN CELL; INTRON; MAMMAL CELL; METHYLATION; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOMATIC CELL; STEM CELL;

3T3 CELLS; ALTERNATIVE SPLICING; ANIMALS; BLOTTING, NORTHERN; CELLS, CULTURED; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; FEMALE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; ISOENZYMES; MALE; MICE; MOLECULAR SEQUENCE DATA; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

MAMMALIA; MURINAE; STAPHYLOCOCCUS PHAGE 3A;

EID: 0037130945     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(02)00976-9     Document Type: Article

References (24)

1. Bird A. DNA methylation patterns and epigenetic memory. Genes Dev. 16:2002;6-21.
2. Chen T., Ueda Y., Xie S., Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J. Biol. Chem. 2002;. in press.
3. Hata K., Okano M., Lei H., Li E. Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice. Development. 129:2002;1983-1993.
4. Hsieh C. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. Mol. Cell. Biol. 19:1999;82111-88218.
5. Hsu D.-W., Lin M.-J., Lee T.-L., Wen S.-C., Chen X., Shen C.-K.J. Two major forms of DNA (cytosine-5) methyltransferase in human somatic tissues. Proc. Natl. Acad. Sci. USA. 96:1999;9751-9756.
6. Jones P.A., Laird P.W. Cancer epigenetics comes of age. Nat. Genet. 21:1999;163-167.
7. Jones P.A., Takai D. The role of DNA methylation in mammalian epigenetics. Science. 293:2001;1068-1070.
8. Lei H., Oh S.P., Okano M., Juttermann R., Goss K.A., Jaenisch R., Li E. De novo cytosine methyltransferase activities in mouse embryonic stem cells. Development. 122:1996;3195-3205.
9. Li E., Bestor T.H., Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell. 69:1992;915-926.
10. Liang G., Chan M., Tomigahara Y., Tsai Y.C., Gonzales F.A., Li E., Laird P.W., Jones P.A. Cooperativity between DNA methyltransferases in the maintenance methylation of repetitive elements. Mol. Cell. Biol. 22:2002;480-491.
11. Mertineit C., Yoder J.A., Taketo T., Laird D.W., Trasler J.M., Bestor T.H. Sex-specific exons control DNA methyltransferase in mammalian germ cells. Development. 125:1998;889-897.
12. Okano M., Xie S., Li E. Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nat. Genet. 19:1998;219-220.
13. Okano M., Bell D.W., Haber D.A., Li E. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 99:1999;247-257.
14. Pesole G., Mignone F., Gissi C., Grillo G., Licciulli F., Liuni S. Structural and functional features of eukaryotic mRNA untranslated regions. Gene. 276:2001;73-81.
15. Pradhan S., Talbot D., Sha M., Benner J., Hornstra L., Li E., Jaenisch R., Roberts R.J. Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase. Nucleic Acids Res. 25:1997;4666-4673.
16. Pradhan S., Bacolla A., Wells R.D., Roberts R.J. Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of de novo and maintenance methylation. J. Biol. Chem. 274:1999;33002-33010.
17. Robertson K.D. DNA methylation, methyltransferases and cancer. Oncogene. 20:2001;3139-3155.
18. Robertson K.D., Uzvolgy E., Liang G., Talmadge C., Sumegi J., Gonzales F.A., Jones P.A. The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nucleic Acids Res. 27:1999;2291-2298.
19. Saito Y., Kanai Y., Sakamoto M., Saito H., Ishii H., Hirohashi S. Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis. Proc. Natl. Acad. Sci. USA. 99:2002;10060-10065.
20. Stewart C., Stuhlmann H., Jahner D., Jaenisch R. De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells. Proc. Natl. Acad. Sci. USA. 79:1982;4098-4102.
21. Takai D., Jones P.A. Comprehensive analysis of CpG islands in human chromosomes 21 and 22. Proc. Natl. Acad. Sci. USA. 99:2002;3740-3745.
22. Velicescu M., Weisenberger D.J., Gonzales F.A., Tsai Y.C., Nguyen C.T., Jones P.A. Cell division is required for de novo methylation of CpG islands in bladder cancer cells. Cancer Res. 62:2002;2378-2384.
23. Xie S., Wang Z., Okano M., Nogami M., Li Y., He W.-W., Okumura K., Li E. Cloning, expression and chromosome locations of the human DNMT3 gene family. Gene. 236:1999;87-95.
24. Yoder J.A., Soman N.S., Verdine G.L., Bestor T.H. DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe. J. Mol. Biol. 270:1997;385-395.