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Volumn 41, Issue 37, 2002, Pages 11126-11133

Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme

(8) Nguyen, Tien V a Riggs, Charles b Babovic Vuksanovic, Dusica a Kim, Yong Sung c Carpenter, John F c Burghardt, Thomas P a Gregersen, Niels d Vockley, Jerry a

a MAYO CLINIC (United States)

b UNIVERSITY OF ARKANSAS (United States)

c UNIVERSITY OF COLORADO (United States)

d AARHUS UNIVERSITY HOSPITAL (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

INCUBATION;

CATALYSIS; FATTY ACIDS; FLUORESCENCE; GENES; MUTAGENESIS; PURIFICATION; SPECTROSCOPY;

ENZYMES;

ACYL COENZYME A DEHYDROGENASE; BACTERIAL PROTEIN; CHAPERONIN; ENZYME VARIANT; GUANIDINE; MUTANT PROTEIN;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME POLYMORPHISM; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; FLUORESCENCE; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; ULTRAVIOLET SPECTROSCOPY; WILD TYPE;

ACYL-COA DEHYDROGENASE; ACYL-COA DEHYDROGENASES; AMINO ACID SUBSTITUTION; ARGININE; CATALYSIS; CIRCULAR DICHROISM; ENZYME STABILITY; ESCHERICHIA COLI; FATTY ACIDS, VOLATILE; GLYCINE; GROEL PROTEIN; GROES PROTEIN; GUANIDINE; HEAT; HUMANS; OXIDATION-REDUCTION; POLYMORPHISM, GENETIC; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SERINE; SPECTROPHOTOMETRY; TRYPTOPHAN; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PRIACANTHUS HAMRUR; PROKARYOTA;

EID: 0037125921 PISSN: 00062960 EISSN: None Source Type: Journal
DOI: 10.1021/bi026030r Document Type: Article

Times cited : (32)

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