Optimizing the enzymatic determination of galactose in the culture medium of rat liver and HepG2 cell spheroids

Analytical Biochemistry

Volumn 311, Issue 2, 2002, Pages 179-181

  Xu, Jinsheng ^a   Ma, Mingwen ^a   Purcell, Wendy M ^a  

^a UNIVERSITY OF THE WEST OF ENGLAND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

CULTURE MEDIUM; ENZYMATIC DETERMINATION; HEP-G2 CELLS; RAT LIVERS;

CHEMISTRY;

GALACTOSE; GALACTOSE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PLASMA PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ANIMAL TISSUE; ARTICLE; CATALYSIS; CONCENTRATION RESPONSE; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME KINETICS; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER; NONHUMAN; PH; PRIORITY JOURNAL; RAT; SPHEROID CELL; ANIMAL; CELL CULTURE; CYTOLOGY; ENZYMOLOGY; KINETICS; METABOLISM;

ANIMALIA;

ANIMALS; CULTURE MEDIA, CONDITIONED; GALACTOSE; GALACTOSE DEHYDROGENASES; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; LIVER; NAD; RATS; TUMOR CELLS, CULTURED;

EID: 0037114943     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00400-1     Document Type: Article

References (4)

1. H. Varley, A.H. Gowenlock, M. Bell, Chapter 29: Tests in liver and biliary tract diseases, in: Practical Clinical Biochemistry, Fifth ed., William Heinemann Medical Books Ltd, London, 1980, pp. 1051-1054.
2. A. Val, J.F. Huidobro, M.P. Sánchez, S. Muniategui, M.A. Fernandez-Muino, M.T. Sancho, Enzymatic determination of galactose and lactose in honey, J. Agric. Food Chem. 46 (1998) 1381-1385.
3. M. Doudoroff, C.R. Contopoulou, S. Burns, in: Proc. Int. Symp. Enzyme Chem. Tokyo-Kyoto 1957, Academic Press, New York, 1958, p. 313.
4. B.B. Knoweles, C.C. Howe, D.P. Aden, Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen, Science 209 (1980) 497-499.