A multidonor ELISPOT study of IL-1β, IL-2, IL-4, IL-6, IL-13, IFN-γ and TNF-α release by cryopreserved human peripheral blood mononuclear cells

Journal of Immunological Methods

Volumn 270, Issue 2, 2002, Pages 171-182

  Bailey, Tanya ^a   Stark, Sarah ^a   Grant, Angela ^a   Hartnett, Christopher ^a   Tsang, Monica ^a   Kalyuzhny, Alexander ^a  

^a R and D Systems Inc   (United States)

Author keywords

ELISPOT; Frozen PBMC; Optimization; TH1 TH2 cytokines

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 13; INTERLEUKIN 1BETA; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 6; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BLOOD DONOR; CONTROLLED STUDY; CRYOPRESERVATION; CYTOKINE PRODUCTION; ENZYME LINKED IMMUNOSPOT ASSAY; FREEZING; HUMAN; HUMAN CELL; IMMUNOASSAY; MONONUCLEAR CELL; NORMAL HUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; THAWING;

CRYOPRESERVATION; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; INTERFERON TYPE II; INTERLEUKIN-1; INTERLEUKIN-13; INTERLEUKIN-2; INTERLEUKIN-4; INTERLEUKIN-6; LEUKOCYTES, MONONUCLEAR; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0037114289     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00297-1     Document Type: Article

References (18)

1. Asai T., Storkus W.J., Whiteside T.L. Evaluation of the modified ELISPOT assay for gamma interferon production in cancer patients receiving antitumor vaccines. Clin. Diagn. Lab. Immunol. 2:2000;145.
2. Canaan T., D'Alesandro M.M., Miller G.A. Cytokine production by cryopreserved human peripheral blood mononuclear cells in response to periodontal pathogens. Arch. Oral Biol. 8:1998;657.
3. Ekerfelt C., Ernerudh J., Jenmalm M.C. Detection of spontaneous and antigen-induced human interleukin-4 responses in vitro: comparison of ELISPOT, a novel ELISA and real-time RT-PCR. J. Immunol. Methods. 260:2002;55.
4. Eriksson K., Nordstrom I., Horal P., Jeansson S., Svennerholm B., Vahlne A.et al. Amplified ELISPOT assay for the detection of HIV-specific antibody-secreting cells in subhuman primates. J. Immunol. Methods. 1-2:1992;107.
5. Howell D.M., Feldman S.B., Kloser P., Fitzgerald-Bocarsly P. Decreased frequency of functional natural interferon-producing cells in peripheral blood of patients with the acquired immune deficiency syndrome. Clin. Immunol. Immunopathol. 2:1994;223.
6. Kabilan L., Andersson G., Lolli F., Ekre H.P., Olsson T., Troye-Blomberg M. Detection of intracellular expression and secretion of interferon-gamma at the single-cell level after activation of human T cells with tetanus toxoid in vitro. Eur. J. Immunol. 5:1990;1085.
7. Kalyuzhny A., Stark S. A simple method to reduce the background and improve well-to-well reproducibility of staining in ELISPOT assays. J. Immunol. Methods. 257:2001;93.
8. Kamath A.T., Groat N.L., Bean A.G., Britton W.J. Protective effect of DNA immunization against mycobacterial infection is associated with the early emergence of interferon-gamma (IFN-gamma)-secreting lymphocytes. Clin. Exp. Immunol. 3:2000;476.
9. Keane N.M., Price P., Stone S.F., John M., Murray R.J., French M.A. Assessment of immune function by lymphoproliferation underestimates lymphocyte functional capacity in HIV patients treated with highly active antiretroviral therapy. AIDS Res. Hum. Retrovir. 18:2000;1991.
10. Lewalle P., Rouas R., Lehmann F., Martiat P. Freezing of dendritic cells, generated from cryopreserved leukaphereses, does not influence their ability to induce antigen-specific immune responses or functionally react to maturation stimuli. J. Immunol. Methods. 240:2000;69.
11. McCutcheon M., Wehner N., Wensky A., Kushner M., Doan S., Hsiao L.et al. A sensitive ELISPOT assay to detect low-frequency human T lymphocytes. J. Immunol. Methods. 210:1997;149.
12. Merker R., Check I., Hunter R.L. Use of cryopreserved cells in quality control of human lymphocyte assays: analysis of variation and limits of reproducibility in long-term replicate studies. Clin. Exp. Immunol. 1:1979;116.
13. Pass H.A., Schwarz S.L., Wunderlich J.R., Rosenberg S.A. Immunization of patients with melanoma peptide vaccines: immunologic assessment using the ELISPOT assay. Cancer J. Sci. Am. 5:1998;316.
14. Schmittel A., Keilholz U., Thiel E., Scheibenbogen C. Quantification of tumor-specific T lymphocytes with the ELISPOT assay. J. Immunother. 3:2000;289.
15. Smith J.G., Liu X., Kaufhold R.M., Clair J., Caulfield M.J. Development and validation of a gamma interferon elispot assay for quantitation of cellular immune responses to varicella-zoster virus. Clin. Diagn. Lab. Immunol. 5:2001;871.
16. Sobota V., Bubenik J., Indrova M., Vlk V., Jakoubkova J. Use of cryopreserved lymphocytes for assessment of the immunological effects of interferon therapy in renal cell carcinoma patients. J. Immunol. Methods. 203:1997;1.
17. Tanguay S., Killion J.J. Direct comparison of ELISPOT and ELISA-based assays for detection of individual cytokine-secreting cells. Lymphokine Cytokine Res. 4:1994;259.
18. Weinberg A., Wohl D.A., Brown D.G., Pott G.B., Zhang L., Ray M.G.et al. Effect of cryopreservation on measurement of cytomegalovirus-specific cellular immune responses in HIV-infected patients. J. Acquir. Immune Defic. Syndr. 2:2000;109.