Two-dimensional separation of the membrane protein sarcoplasmic reticulum Ca-ATPase for high-performance liquid chromatography-tandem mass spectrometry analysis of posttranslational protein modifications

Analytical Biochemistry

Volumn 308, Issue 2, 2002, Pages 328-335

  Sharov, Victor S ^a   Galeva, Nadezhda A ^a   Knyushko, Tatyana V ^a   Bigelow, Diana J ^a   Williams, Todd D ^a   Schöneich, Christian ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROSPRAY IONIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLAR RATIO; MUSCLE;

ELECTROSPRAY IONIZATION MASS SPECTROMETRY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRIES; MATRIX ASSISTED LASER DESORPTION IONIZATION; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN MODIFICATIONS; REVERSED PHASE HPLC; SARCOPLASMIC RETICULUM; TWO-DIMENSIONAL SEPARATIONS;

PROTEINS;

3 NITROTYROSINE; ADENOSINE TRIPHOSPHATASE (CALCIUM); MUSCLE ENZYME;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; ENZYME PURIFICATION; FAST MUSCLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; RABBIT;

ORYCTOLAGUS CUNICULUS;

EID: 0037108986     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00261-0     Document Type: Article

References (17)

1. E.R. Stadtman, Protein oxidation and aging, Science 257 (1992) 1220-1224.
2. E.R. Stadtman, R.L. Levine, Protein oxidation, Ann. N. Y. Acad. Sci. 899 (2000) 191-208.
3. R.I. Viner, D.A. Ferrington, A.F.R. Hühmer, D.J. Bigelow, C. Schóneich, Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca-ATPase of rat skeletal muscle during aging: a peroxynitrite-mediated process?, FEBS Lett. 379 (1996) 286-290.
4. R.I. Viner, D.A. Ferrington, T.D. Williams, D.J. Bigelow, C. Schöneich, Protein modification during biological aging: selective tyrosine nitration of the SERCA2a isoform of the sarcoplasmic reticulum Ca-ATPase in rat skeletal muscle, Biochem. J. 340 (1999) 657-669.
5. 2+-ATPase function and lowers tyrosine nitration in hypercholesterolemia and improves nitric oxide-induced relaxation, Circ. Res. 90 (2002) 1114-1121.
6. R.I. Viner, T.D. Williams, C. Schösneich, Nitric oxide-dependent modification of the sarcoplasmic reticulum Ca-ATPase: localization of cysteine target sites, Free Radical Biol. Med. 29 (2000) 489-496.
7. H. Ischiropoulos, A.B. Al-Mehdi, Peroxynitrite-mediated oxidative protein modifications, FEBS Lett. 364 (1995) 279-282.
8. S.A.B. Greenacre, H. Ischiropoulos, Tyrosine nitration: localization, quantification, consequences for protein function and signal transduction, Free Rad. Res. 34 (2001) 541-581.
9. S.P. Gygi, G.L. Corthals, Y. Zhang, Y. Rochon, R. Aebersold, Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology, Proc. Natl. Acad. Sci. USA 97 (2000) 9390-9395.
10. M.P. Molloy, Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients, Anal. Biochem. 280 (2000) 1-10.
11. J.L. Fernandez, M. Rosemblatt, C. Hidalgo, Highly purified sarcoplasmic reticulum vesicles are devoid of Ca2+-independent ('basal') ATPase activity, Biochim. Biophys. Acta 599 (1980) 522-568.
12. W.A. Pryor, R. Cueto, X. Jin, W.H. Koppenol, M. Ngu-Schwemlein, G.I. Squadrito, P.L. Uppu, R.M. Uppu, A practical method for preparing peroxynitrite solutions of low ionic strength and free of hydrogen peroxide, Free Radical Biol. Med. 18 (1995) 75-83.
13. M.R. Sussman. Purification of integral membrane proteins by reverse-phase high performance liquid chromatography, Anal. Biochem. 169 (1987) 395-399.
14. K.D. Speicher, O. Kolbas, S. Harper, D.W. Speicher, Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies, J. Biomol. Techniques 11 (2000) 74-86.
15. P. Roepstorff, J. Fohlman, Proposal for a common nomenclature for sequence ions in mass spectra of peptides, Biomed. Mass Spectrom. 11 (1984) 601.
16. C.J. Brandl, N.M. Green, B. Korczak, D.H. MacLennan, Two Ca-ATPase genes: homologies and mechanistic implications of deduced amino acid sequences, Cell 44 (1986) 597-607.
17. 2+-ATPase by peroxynitrite, Free Rad. Res. 24 (1996) 243-259.