Direct determination of tryptophan using high-performance anion-exchange chromatography with integrated pulsed amperometric detection

Analytical Biochemistry

Volumn 308, Issue 2, 2002, Pages 204-209

  Hanko, Valoran P ^a   Rohrer, Jeffrey S ^a  

^a DIONEX CORPORATION   (United States)

Author keywords

AAA Direct; Amino acid; Animal feed; Anion exchange chromatography; Bacteria; Cell culture; Escherichia coli; Fermentation broth; HPAE; HPLC; Integrated pulsed amperometric detection; Ion exchange chromatography; IPAD; Media; PAD; Tryptophan; Yeast

Indexed keywords

AMINO ACIDS; BACTERIA; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; FERMENTATION; ION CHROMATOGRAPHY; ION EXCHANGE; IONS; MAMMALS; PROTEINS; SODIUM HYDROXIDE; YEAST;

AAA-DIRECT; ANIMAL FEED; ANION EXCHANGE CHROMATOGRAPHY; FERMENTATION BROTHS; HPAE; HPLC; INTEGRATED PULSED AMPEROMETRIC DETECTION; ION EXCHANGE CHROMATOGRAPHY; IPAD; MEDIA; TRYPTOPHAN;

ANIMAL CELL CULTURE;

AMINO ACID; BOVINE SERUM ALBUMIN; PROTEIN; SODIUM HYDROXIDE; TRYPTOPHAN;

ACCURACY; ALKALINITY; AMINO ACID ANALYSIS; AMINO ACID COMPOSITION; AMPEROMETRY; ANIMAL FOOD; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CELL CULTURE; ESCHERICHIA COLI; FERMENTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; INJECTION; ION EXCHANGE CHROMATOGRAPHY; PRIORITY JOURNAL; YEAST;

ANIMALIA; BOVINAE; ESCHERICHIA COLI;

EID: 0037108920     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00215-4     Document Type: Article

References (47)

1. T.W. Goodwin, R.A. Morton, The spectrophotometric determination of tyrosine and tryptophan in proteins, Biochem. J. 40 (1946) 628-632.
2. H. Edelhoch, Spectroscopic determination of tryptophan and tyrosine in proteins, Biochemistry 6 (1967) 1948-1954.
3. W.L. Bencze, K. Schmid, Determination of tyrosine and tryptophan in proteins, Anal. Chem. 29 (1957) 1193-1196.
4. P.J. Bredderman, Tryptophan analysis of proteins in 6 M guanidine hydrochloride: modification for more general application, Anal. Biochem. 61 (1974) 298-301.
5. J.R. Spies, D.C. Chambers, Chemical determination of tryptophan, Anal. Chem. 20 (1948) 30-39.
6. T.E. Barman, D.E. Koshland Jr., A colorimetric procedure for the quantitative determination of tryptophan residues in proteins, J. Biol. Chem. 242 (1967) 5771-5776.
7. D. Fukushima, Internal structure of 7S and 11S globulin molecules in soy bean proteins, Cereal Chem. 45 (1968) 203-224.
8. G. Barth, R. Records, E. Bunnenberg, C. Djerassi, W. Voelter, Magnetic circular dichroism studies. XII. The determination of tryptophan in proteins, J. Am. Chem. Soc. 93 (1971) 2545-2547.
9. M.C. DeTraglia, J.S. Brand, A.M. Tometsko, The reaction of 3-diazonium-1,2,4-triazole with tryptophan at acid pH: a scintillation method for tryptophan determination in proteins, Anal. Biochem. 99 (1979) 464-473.
10. H. Matsubara, R.M. Sasaki, High recovery of tryptophan from acid hydrolysates of proteins, Biochem. Biophys. Res. Commun. 35 (1969) 175-181.
11. M.L. Bozzini, R. Bello, N. Cagle, D. Yamane, D. Dupont, Tryptophan recovery from autohydrolyzed samples using dodecanethiol, Appl. Biosyst. Res. News, February, 1991.
12. S. Moore, W.H. Stein, Chromatographic determination of amino acids by the use of automatic recording equipment, Methods Enzymol. 6 (1963) 819-831.
13. J.W. Crabb, K.A. West, W.S. Dodson, J.D. Hulmes, in: Amino Acid Analysis. Current Protocols in Protein Science, Wiley, New York, 1997, pp. 11.9.1-11.9.42.
14. B.A. Bidlingmeyer, T.L. Tarvin, S.A. Cohen, Amino acid analysis of submicrogram hydrolysate samples, in: Methods in Protein Sequence Analysis, Humana, Clifton, NJ, 1986, pp. 229-244.
15. T.-Y. Liu, Y.H. Chang, Hydrolysis of proteins with p-toluene-sulfonic acid. Determination of tryptophan, J. Biol. Chem. 246 (1971) 2842-2848.
16. R.J. Simpson, M.R. Neuberger, T.-Y. Liu, Complete amino acid analysis of proteins from a single hydrolysate, J. Biol. Chem. 251 (1976) 1936-1940.
17. B. Penke, R. Ferenezi, K. Kovacs, A new acid hydrolysis method for determining tryptophan in peptides and proteins, Anal. Biochem. 60 (1974) 45-50.
18. L.K. Creamer, A.R. Matheson, The use of mercaptoethanesulfonic acid as a hydrolyzing agent for the determination of tryptophan in proteins, N. Z. J. Dairy Sci. Tech. 11 (1976) 211-212.
19. R.L. Hill, W.R. Schmidt, The complete enzymic hydrolysis of proteins, J. Biol. Chem. 237 (1962) 389-396.
20. T. Sasaki, B. Abrams, B.L. Horecker, A fluorometric method for the determination of the tryptophan content of proteins, Anal. Biochem. 65 (1975) 396-404.
21. J.R. Spies, Determination of tryptophan in proteins, Anal. Chem. 39 (1967) 1412-1416.
22. N.D. Danielson, L.B. Rogers, Determination of tryptophan in proteins by pyrolysis gas chromatography, Anal. Chem. 50 (1978) 1680-1683.
23. A. Homer, A method for the determination of the tryptophane content of proteins, involving the use of baryta as hydrolyzing reagent, J. Biol. Chem. 22 (1915) 369-389.
24. J.W.H. Lugg, Investigations of sources of error in the estimation of tyrosine and tryptophan in complex materials, which are associated with hydrolysis, Biochem. J. 32 (1938) 775-783.
25. A. Dreze, Le doasage du tryptophane dans les milieux naturels. II. - La stabilité du tryptophane au cours de L' hydrolyse alkaline effectuée en présence D' hydrates de carbone, Bull. Soc. Chim. Biol. 42 (1960) 407-417.
26. F.J. Oelshlegel Jr., J.R. Schroeder, M.A. Stahmann, A simple procedure for basic hydrolysis of proteins and rapid determination of tryptophan using a starch column, Anal. Biochem. 34 (1970) 331-337.
27. E.J. Robel, Ion-exchange chromatography for the determination of tryptophan, Anal. Biochem. 18 (1967) 406-413.
28. D.H. Spackman, W.H. Stein, S. Moore, Automatic recording apparatus for use in the chromatography of amino acids, Anal. Chem. 30 (1958) 1190-1206.
29. E.A. Noltmann, T.A. Mahowald, S.A. Kuby, Studies on adenosine triphosphate transphosphorylases. II. Amino acid composition of adenosine triphosphate-creatine transphoshorylase, J. Biol. Chem. 237 (1962) 1146-1154.
30. R. Knox, G.O. Kohler, R. Palter, H.G. Walker, Determination of tryptophan in feeds, Anal. Biochem. 36 (1970) 136-143.
31. D.H. Spackman, Accelerated system for the automatic analysis of amino acids, Fed. Proc. 22 (1963) 244.
32. K.A. Walsh, J.R. Brown, Internal standards for amino acid analysis, Biochim. Biophys. Acta 58 (1962) 596-598.
33. M. Wilkinson, G.A. Iacobucci, D.V. Myers, 5-Methyltyrptophan: an internal standard for tryptophan determination by ion-exchange chromatography, Anal. Biochem. 70 (1976) 470-478.
34. T.E. Hugli, S. Moore, Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates, J. Biol. Chem. 247 (1972) 2828-2834.
35. S. Moore, Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction, J. Biol. Chem. 243 (1968) 6281-6283.
36. P. Slump, H.A.W. Schreuder, Determination of tryptophan in foods, Anal. Biochem. 27 (1969) 182-185.
37. K.-T. Hsu, B.L. Currie, High-performance liquid chromatography of Dns-amino acids and application to peptide hydrolysates, J. Chromatogr. 166 (1978) 555-561.
38. P. Lindroth, K. Mopper, High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde, Anal. Chem. 51 (1979) 1667-1674.
39. G.B. Irvine, Amino acid analysis. Precolumn derivatization methods, in: B.J. Smith (Ed.), Methods in Molecular Biology: Protein Sequencing Protocols, vol. 64, Humana Press, Totowa, NJ, 1997, pp. 131-138.
40. AOAC Official Method 988.15, Tryptophan in Foods and Food and Feed Ingredients, Ion Exchange Chromatography Method, AOAC Official Methods Analysis 45.4.04, 1995, p. 66 (Chapter 45).
41. A.J. Smith, Postcolumn amino acid analysis, in: B.J. Smith (Ed.), Methods in Molecular Biology: Protein Sequencing Protocols, vol. 64, Humana Press, Totowa, NJ, 1997, pp. 139-146.
42. A.P. Clarke, P. Jandik, R.D. Rocklin, Y. Liu, N. Avdalovic, An integrated amperometry waveform for the direct, sensitive detection of amino acids and amino sugars following anion-exchange chromatography, Anal. Chem. 71 (1999) 2774-2781.
43. P. Jandik, A.P. Clarke, N. Avdalovic, D.C. Andersen, J. Cacia, Analyzing mixtures of amino acids and carbohydrates using bimodal integrated amperometric detection, J. Chromatogr. B 732 (1999) 193-201.
44. Dionex Corporation. Installation Instructions and Troubleshooting Guide for the AAA-Direct Amino Acid Analysis System. Document No. 031481, Revision 03, April 6, Dionex Corporation, Sunnyvale, CA, 2000.
45. K. Hirayama, S. Akashi, M. Furuya, K. Fukuhara, Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and Frit-FAB LC/MS, Biochem. Biophys. Res. Commun. 173 (1990) 639-646.
46. Dionex Corporation. Technical Note 50, Determination of the amino acid content of peptides by AAA-Direct Dionex Corporation, Sunnyvale, CA, 2000.
47. V.P. Hanko, J.S. Rohrer, Determination of carbohydrates, sugar alcohols, and glycols in cell cultures and fermentation broths using high-performance anion-exchange chromatography with pulsed amperometric detection, Anal. Biochem. 283 (2000) 192-199.