Quantitation of repetitive epitopes in glycosaminoglycans immobilized on hydrophobic membranes treated with cationic detergents

Analytical Biochemistry

Volumn 308, Issue 2, 2002, Pages 210-222

  Rosén, Madeleine ^a   Edfors Lilja, Inger ^a   Björnsson, Sven ^b  

^a LINNAEUS UNIVERSITY   (Sweden)

^b LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Alcian blue; Blotting; Cationic detergent; Chondroitin sulfate; Dermatan sulfate; Dot blot; Epitopes; Glycosaminoglycan; Heparan sulfate; Immobilization; Membrane bound; Proteoglycan; Sequences; Solid phase; Transfer

Indexed keywords

GLYCOLS; GLYCOPROTEINS; HYDROPHOBICITY; MONOCLONAL ANTIBODIES; RADIOACTIVE WASTE VITRIFICATION; SOAPS (DETERGENTS); SULFUR COMPOUNDS;

ALCIAN BLUES; BLOTTING; CATIONIC DETERGENT; CHONDROITIN SULFATES; DERMATAN SULFATE; DOT BLOTS; GLYCOSAMINOGLYCANS; HEPARAN SULFATES; MEMBRANE-BOUND; PROTEOGLYCANS; SEQUENCES; SOLID-PHASE; TRANSFER;

EPITOPES;

AGAROSE; ALCIAN BLUE; AMINOSUGAR; ANTIBODY; BUFFER; CATION; CHONDROITIN SULFATE; DERMATAN SULFATE; DETERGENT; DIETHYLENE GLYCOL; EPITOPE; GLUCURONIC ACID; GLYCOSAMINOGLYCAN; HEPARAN SULFATE; MONOCLONAL ANTIBODY; N ACETYLGALACTOSAMINE 4 SULFATASE; POLYMER; PROTEOGLYCAN; SULFATE; URONIC ACID;

AGAR GEL ELECTROPHORESIS; ANTIGEN RECOGNITION; ARTICLE; CARBOHYDRATE ANALYSIS; CHEMICAL COMPOSITION; DENSITY; DOT HYBRIDIZATION; HYDROPHOBICITY; IMMOBILIZATION; IMMUNOBLOTTING; MEMBRANE BINDING; PRIORITY JOURNAL; SOLID; STAINING;

EID: 0037108658     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00206-3     Document Type: Article

References (24)

1. B. Caterson, F. Mahmoodian, J.M. Sorrell, T. Hardingham, M.T. Bayliss, S.L. Carney, A. Ratcliffe, H. Muir, Modulation of native chondroitin sulphate structure in tissue development and in disease, J. Cell Sci. 97 (1990) 411-417.
2. J.M. Sorrell, D.A. Carrino, A.I. Caplan, Structural domains in chondroitin sulphate identified by anti-chondroitin sulphate monoclonal antibodies. Immunosequencing of chondroitin sulphates, Matrix 13 (1993) 351-361.
3. T.E. Hardingham, A.J. Fosang, N.J. Hey, P.K. Hazell, W.J. Kee, R.J.F. Ewins, The sulphation pattern in chondroitin sulphate chains investigated by chondroitinase ABC and ACII digestion and reactivity with monoclonal antibodies, Carbohydr. Res. 255 (1994) 241-254.
4. R.D. Rosenberg, N.W. Shworak, J. Liu, J.J. Schwartz, L. Zhang, Cell adhesion in vascular biology: Heparan sulphate proteoglycans of the cardiovascular system. Specific structures emerge but how is synthesis regulated?, J. Clin. Invest. 99 (1997) 2062-2070.
5. A. Ratcliffe, W. Shurety, B. Caterson, The quantitation of a native chondroitin sulphate epitope in synovial fluid lavages and articular cartilage from canine experimental osteoarthritis and disuse atrophy, Arthritis Rheum. 36 (1993) 543-551.
6. G. Rizkalla, A. Reiner, E. Bogoch, R. Poole, Studies on the articular cartilage proteoglycan aggrecan in health and osteoarthritis. Evidence for molecular heterogeneity and extensive molecular changes in disease, J. Clin. Invest. 90 (1992) 2268-2277.
7. V.C. Hascall, R.J. Midura, J.M. Sorrell, A.H. Plaas, Immunology of chondroitin/dermatan sulphate [Review], Adv. Exp. Med. Biol. 376 (1995) 205-216.
8. K. Kuwaba, Y. Nomura, S. Irie, Y. Koyama, Temporal changes in disaccharide composition of dermatan sulfate in the skin after epicutaneous application of hapten, J. Dermatol. Sci. 19 (1999) 23-30.
9. J.M. Sorrell, A.M. Lintala, F. Mahmoodian, B. Caterson, Epitope-specific changes in chondroitin sulphate/dermatan sulphate proteoglycans as markers in the lymphopoietic and granulopoietic compartments of developing bursae of Fabricius, J. Immunol. 140 (1988) 4263-4270.
10. R. Perris, D. Krotoski, T. Lallier, C. Domingo, J.-M. Sorell, M. Bronner-Fraser, Spatial end temporal changes in the distribution of proteoglycans during avian crest development, Development 111 (1991) 583-599.
11. I. Charvet, F.J. Hemming, C. Feuerstein, R. Saxod, Mosaic distribution of chondroitin sulphate and keratan sulphate in the developing rat striatum: Possible involvement of proteoglycans in the organization of the nigrostriatal system, Brain Res. Dev. Brain Res. 109 (1998) 229-244.
12. Y.G. Brickman, M.D. Ford, J.T. Gallagher, V. Nurcombe, P.F. Bartlett, J.E. Turnbull, Structural modification of fibroblast growth factor-binding heparan sulfate at a determinative stage of neural development, J. Biol. Chem. 273 (1998) 4350-4359.
13. M Karlsson, I. Edfors-Lilja, S. Björnsson, Binding and detection of glycosaminoglycans immobilized on membranes treated with cationic detergents, Anal. Biochem. 286 (2000) 51-58.
14. S. Björnsson, Simultaneous preparation and quantitation of proteoglycans by precipitation with Alcian blue, Anal. Biochem. 210 (1993) 282-291.
15. M. Karlsson, S. Björnsson, in: Iozzo (Ed.), Methods in Molecular Biology: Proteoglycan Protocols, vol. 171, Humana Press, Clifton, NJ, 2001, pp. 159-173.
16. S. Björnsson, Quantitation of proteoglycans as glycosaminoglycans in biological fluids using an Alcian blue dot blot analysis, Anal. Biochem. 256 (1998) 229-237.
17. S. Björnsson, Size dependent separation of proteoglycans by electrophoresis in gels of pure agarose, Anal. Biochem 210 (1993) 292-298.
18. C.P. Dietrich, S.M. Dietrich, Electrophoretic behaviour of acidic mucopolysaccharides in diamine buffers, Anal. Biochem. 70 (1976) 645-647.
19. B. Caterson, J.E. Christner, J.R. Baker, J.R. Couchman, Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans, Fed. Proc. 44 (1985) 386-393.
20. J.R. Couchman, B. Caterson, J.E. Christner, J.R. Baker, Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues, Nature 307 (1984) 650-652.
21. Z. Avnur, G. Geiger, Immunocytochemical localization of native chondroitin-sulphate in tissues and cultured cells using a specific monoclonal antibody, Cell 38 (1984) 811-822.
22. S. Kure, O. Yoshie, A syngenic monoclonal antibody to murine meth-A sarcoma (HepSS-1) recognizes heparan sulphate glycosaminoglycan (Hs-GAG): Cell density and transformation dependent alteration in cell surface Hs-GAG defined by HepSS-1, J. Immunol. 137 (1986) 3900-3908.
23. S.L. Carney, B. Caterson, H.R. Penticost, The investigation of glycosaminoglycan mimotope structure using capillary electrophoresis and other complementary electrophoretic techniques, Electrophoresis 17 (1996) 384-390.
24. M. Lyon, J.T. Gallagher, Bio-specific sequences and domains in heparan sulphate and the regulation of cell growth and adhesion, Matrix Biol. 17 (1998) 485-493.