Binding and detection of glycosaminoglycans immobilized on membranes treated with cationic detergents

Analytical Biochemistry

Volumn 286, Issue 1, 2000, Pages 51-58

  Karlsson, Madeleine ^a   Edfors Lilja, Inger ^a   Björnsson, Sven ^b  

^a LINNAEUS UNIVERSITY   (Sweden)

^b CENTRAL HOSPITAL   (Sweden)

Author keywords

Alcian blue; Blotting; Cationic detergent; Chondroitin sulfate; Dermatan sulfate; Dot blot; Epitopes; Glycosaminoglycan; Heparan sulfate; Immobilization; Membrane bound; Proteoglycan; Sequences; Solid phase; Transfer

Indexed keywords

BLOOD; CEREBROSPINAL FLUID; ELECTROPHORESIS; EPITOPES; FLUORINE COMPOUNDS; HYDROPHILICITY; HYDROPHOBICITY; IONIC STRENGTH; MOLECULES; PHASE SEPARATION; RADIOACTIVE WASTE VITRIFICATION; SOAPS (DETERGENTS); SULFUR COMPOUNDS;

ALCIAN BLUES; BLOTTING; CATIONIC DETERGENT; CHONDROITIN SULFATES; DERMATAN SULFATE; DOT BLOTS; GLYCOSAMINOGLYCANS; HEPARAN SULFATES; MEMBRANE-BOUND; PROTEOGLYCANS; SEQUENCES; SOLID-PHASE; TRANSFER;

MEMBRANES;

ALCIAN BLUE; CHONDROITIN 6 SULFATE; DETERGENT; FIBRINOGEN; GLYCOSAMINOGLYCAN; HEPARAN SULFATE; KERATAN SULFATE; POLYVINYLIDENE FLUORIDE; PYROXYLIN; TOLONIUM CHLORIDE;

ANALYTIC METHOD; ARTICLE; CARBOHYDRATE ANALYSIS; HYDROPHOBICITY; IONIC STRENGTH; MEMBRANE BINDING; PRIORITY JOURNAL; SOLID PHASE EXTRACTION;

EID: 0034327851     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4767     Document Type: Article

References (18)

1. Proteoglycans: Structures and interactions
2. Aggrecan immobilization onto polystyrene plates through electrostatic interactions with spermine
3. Detecting proteoglycans immobilized on positively charged nylon
4. Autoantibodies to vascular heparan sulfate proteoglycan in systemic lupus erythematosus react with endothelial cells and inhibit formation of thrombin-antithrombin III complexes
5. Analysis of negatively charged dye-binding antibodies reactive with double-stranded DNA and heparan sulfate in serum from patients with rheumatic diseases
6. Optimization of an Alcian blue dot-blot assay for the detection of glycosaminoglycans and proteoglycans
7. The precipitation of polyanions by long-chain aliphatic ammonium compounds
8. Microsequence analysis of peptides and proteins
9. Effect of polybrene on the N-terminal sequencing of peptides bound to polyvinylidene difluoride membranes
10. Simultaneous preparation and quantitation of proteoglycans by precipitation with Alcian blue
11. Quantitation of proteoglycans as glycosaminoglycans i in biological fluids using an Alcian blue dot blot analysis
12. Size dependent separation of proteoglycans by electrophoresis in gels of pure agarose
13. 125I-cationized cytocrome c of proteoglycans and glycosaminoglycans immobilized on unmodified and positively charged nylon 66
14. Characterization of biotin-labeled proteoglycans by electrophoretic separation on minigels and blotting onto nylon membranes prior and after enzymatic digestion
15. Identification of monoclonal antibodies that recognize novel epitopes in native chondroitin/dermatan sulfate glycosaminoglycan chains: Their use in mapping functionally distinct domains of human skin
16. Characterization of proteoglycan populations separated by agarose electrophoresis
17. Evidence for the presence of a large keratan sulfate proteoglycan in the human uterine cervix
18. Development of an enzyme immunoassay specific for a core protein epitope of a novel small basement membrane associated heparan sulphate proteoglycan from human kidney