A nonradioactive method for the assay of polyphosphate kinase activity and its application in the study of polyphosphate metabolism in Burkholderia cepacia

Analytical Biochemistry

Volumn 308, Issue 2, 2002, Pages 294-299

  Mullan, Alan ^a   Quinn, John P ^a   McGrath, John W ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Nonradioactive assay; Polyphosphate kinase; Toluidine blue

Indexed keywords

BIOCHEMISTRY; METABOLISM;

BURKHOLDERIA CEPACIA; CATALYZED REACTIONS; ITS APPLICATIONS; KINASE ACTIVITY; NONRADIOACTIVE; POLYPHOSPHATE METABOLISM; POLYPHOSPHATES; TOLUIDINE BLUE;

ENZYMES;

PHOSPHOTRANSFERASE; POLYPHOSPHATE; TOLONIUM CHLORIDE;

ANALYTIC METHOD; ARTICLE; BACTERIAL METABOLISM; BIOSYNTHESIS; BURKHOLDERIA CEPACIA; ENZYME ACTIVITY; ENZYME ASSAY; NONHUMAN; PHOSPHATE METABOLISM; PRIORITY JOURNAL;

BURKHOLDERIA; BURKHOLDERIA CEPACIA;

EID: 0037108656     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00249-X     Document Type: Article

References (22)

1. I.S. Kulaev, V.M. Vagabov, T.V. Kulakovskaya, New aspects of inorganic polyphosphate metabolism and function, J. Biosci. Bioeng. 88 (1999) 111-129.
2. U. Pick, M. Bental, E. Chitlaru, M. Weiss, Polyphosphate-hydrolysis-a protective mechanism against alkaline stress, FEBS Lett. 274 (1990) 15-18.
3. I.S. Kulaev, The Biochemistry of Inorganic Polyphosphates, Wiley, New York, 1979.
4. M. Akiyama, E. Crooke, A. Kornberg, The polyphosphate kinase gene of Escherichia coli-isolation and sequence of the ppk gene and membrane location of the protein, J. Biol. Chem. 267 (1992) 22556-22561.
5. W. Geißdörfer, A. Ratajczak, W. Hillen, Transcription of ppk from Acinetobacter sp. strain ADPI, encoding a putative polyphosphate kinase, is induced by phosphate starvation, Appl. Environ. Microbiol. 64 (1998) 896-901.
6. J. Kato, T. Yamamoto, K. Yamada, H. Ohtake, Cloning sequence and characterisation of the polyphosphate kinase-encoding gene (ppk) of Klebsiella aerogenes, Gene 137 (1993) 242.
7. C.R. Tinsley, E.C. Gotschlich, Cloning and characterization of the meningococcal polyphosphate kinase gene-production of polyphosphate synthesis mutants, Infect. Immun. 63 (1995) 1624-1630.
8. P.L. Trelstad, P. Purdhani, W. Geißdörfer, W. Hillen, J.D. Keasling, Polyphosphate kinase of Acinetobacter sp. Strain ADPI: purification and characterization of the enzyme and its role during changes in extracellular phosphate levels, Appl. Environ. Microbiol. 65 (1999) 3780-3786.
9. C.R. Tinsley, B.N. Manjula, E.C. Gotschlich, Purification and characterization of polyphosphate kinase from Neisseria meningitidis, Infect. Immun. 61 (1993) 3703-3710.
10. N.N. Rao, M.F. Roberts, A. Torriani, Amount and chain length of polyphosphates in Escherichia coli depend on cell growth conditions, J. Bacteriol. 162 (1985) 242-247.
11. M. Streichan, G. Schon, Periplasmic and intracytoplasmic polyphosphate and easily washable phosphate in pure cultures of sewage bacteria, Water Res. 25 (1991) 9-13.
12. A. Kornberg, N.N. Rao, D. Ault-Riche, Inorganic polyphosphate: a molecule of many functions, Annu. Rev. Biochem. 68 (1999) 89-121.
13. J.M. Leitão, B. Lorenz, N. Bachinski, C. Wilhelm, W.E.G. Muller, H.C. Schroder, Osmotic-stress-induced synthesis and degradation of inorganic polyphosphates in the alga Phaeodactylum tricornutum, Mar. Ecol. Progr. Ser. 121 (1995) 279-288.
14. J.W. McGrath, J.P. Quinn, Intracellular accumulation of polyphosphate by the yeast Candida humicola G-1 in response to acid pH, Appl. Environ. Microbiol. 66 (2000) 4068-4073.
15. A. Kornberg, C.D. Fraley, Inorganic polyphosphate: a molecular fossil come to life, ASM News 66 (2000) 275-280.
16. 4-tetraphosphate α, β-phosphorylase, J. Biol. Chem. 270 (1995) 19377-19382.
17. N.N. Rao, S. Liu, A. Kornberg, Inorganic polyphosphate in Escherichia coli: the phosphate regulon and the stringent response, J. Bacteriol. 180 (1998) 2186-2193.
18. D. Ault-Riche, C.D. Fraley, C.M. Tzeng, A. Kornberg, Novel assay reveals multiple pathways regulating stress-induced accumulations of inorganic polyphosphate in Escherichia coli, J. Bacteriol. 180 (1998) 1841-1847.
19. F.M. Harold, Inorganic polyphosphate in biology: structure metabolism, and function, Bacteriol. Rev. 30 (1966) 772-794.
20. N.A. Robinson, H.G. Wood, Polyphosphate kinase from Propionibacterium shermanii-demonstration that the synthesis and utilization of polyphosphate is by a processive mechanism, J. Biol. Chem. 261 (1986) 4481-4485.
21. K.H. Ahn, A. Kornberg, Polyphosphate kinase from Escherichia coli-purification and demonstration of a phosphoenzyme intermediate, J. Biol. Chem. 265 (1990) 11734-11739.
22. K. Murata, T. Uchida, J. Kato, I. Chibata, Polyphosphate kinase: distribution, some properties and its applicafion as an ATP regeneration system, Agric. Biol. Chem. 52 (1988) 1471-1477.