Determination of protein phosphorylation in FcεRI-activated human mast cells by immunoblot analysis requires protein extraction under denaturing conditions

Journal of Immunological Methods

Volumn 268, Issue 2, 2002, Pages 239-243

  Tkaczyk, Christine ^a   Metcalfe, Dean D ^a   Gilfillan, Alasdair M ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Fc RI; Human mast cells; Immunoblot analysis; Protein phosphorylation; Signal transduction

Indexed keywords

IMMUNOGLOBULIN E ANTIBODY; IMMUNOGLOBULIN E RECEPTOR; IMMUNOGLOBULIN FC FRAGMENT; MITOGEN ACTIVATED PROTEIN KINASE; PROTEINASE INHIBITOR;

ACCURACY; ANTIBODY ISOLATION; ANTIBODY SPECIFICITY; ARTICLE; CELL ACTIVATION; CONTROLLED STUDY; EXTRACTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MAST CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN PHOSPHORYLATION; PROTEINASE INHIBITION; SIGNAL TRANSDUCTION;

HUMANS; IMMUNOBLOTTING; MAST CELLS; PHOSPHORYLATION; PROTEIN DENATURATION; PROTEINS; RECEPTORS, IGE;

EID: 0037107489     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00210-7     Document Type: Article

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