A method for detection of overoxidation of cysteines: Peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress

Biochemical Journal

Volumn 366, Issue 3, 2002, Pages 777-785

  Wagner, Elsa ^a   Luche, Sylvie ^a   Penna, Lucia ^a   Chevallet, Mireille ^a   Van Dorsselaer, Alain ^a   Leize Wagner, Emmanuelle ^a   Rabilloud, Thierry ^a  

^a CEA GRENOBLE   (France)

Author keywords

Cysteic acid; Mass spectrometry (MS); Post translational modification; Proteomics

Indexed keywords

BIOMARKERS; ELECTROPHORESIS; ENZYMES; OXIDATION; PHYSIOLOGY; PROTEINS;

OXIDATIVE STRESSES;

BIOCHEMISTRY;

CYSTEIC ACID; CYSTEINE; CYSTEINE SULFINIC ACID; PEROXIREDOXIN;

ARTICLE; CELL DAMAGE; CONTROLLED STUDY; ENZYME ACTIVE SITE; HELA CELL; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN POLYMORPHISM; TWO DIMENSIONAL ELECTROPHORESIS;

AMINO ACID SEQUENCE; BINDING SITES; CYSTEINE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HELA CELLS; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; OXIDATIVE STRESS; OXYGEN; PEPTIDES; PEROXIDASES; PROTEIN ISOFORMS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SULFINIC ACIDS; SULFONIC ACIDS;

EID: 0037106326     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20020525     Document Type: Article

References (31)

1. Sies, H. (1993) Strategies of antioxidant defense. Eur. J. Biochem. 215, 213-219
2. Fridovich, I. and Freeman, B. (1986) Antioxidant defenses in the lung. Annu. Rev. Physiol. 48, 693-702
3. Choi, H. J., Kang, S. W., Yang, C. H., Rhee, S. G. and Ryu, S. E. (1998) Crystal structure of a novel human peroxidase enzyme at 2.0 Å resolution. Nat. Struct. Biol. 5, 400-406
4. Hirotsu, S., Abe, Y., Okada, K., Nagahara, N., Hori, H., Nishino, T. and Hakoshima, T. (1999) Crystal structure of a multifunctional peroxiredoxin heme-binding protein 23 kDa/proliferation-associated gene product. Proc. Natl. Acad. Sci. U.S.A. 96, 12333-12338
5. Declercq, J. P., Evrard, C., Clippe, A., Stricht, D. V., Bernard, A. and Knoops, B. (2001) Crystal structure of human peroxiredoxin 5, a novel type of mammalian peroxiredoxin at 1.5 A resolution. J. Mol. Biol. 311, 751-759
6. Chae, H. Z., Chung, S. J. and Rhee, S. G. (1994) Thioredoxin-dependent peroxidase from yeast. J. Biol. Chem. 269, 27670-27678
7. Kwon, S. J., Park, J. W., Choi, W. K., Kim, I. H. and Kim, K. (1994) Inhibition of metal-catalyzed oxidation systems by a yeast protector protein in the presence of thioredoxin. Biochem. Biophys. Res. Commun. 201, 8-15
8. Chae, H. Z., Kang, S. W. and Rhee, S. G. (1999) Isoforms of mammalian peroxiredoxin that reduce peroxides in presence of thioredoxin. Methods Enzymol. 300, 219-227
9. Matsumoto, A., Okado, A., Fujii, T., Fujii, J., Egashira, M., Niikawa, N. and Taniguchi, N. (1999) Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. FEBS Lett. 443, 246-250
10. Knoops, B., Clippe, A., Bogard, C., Arsalane, K., Wattiez, R., Hermans, C., Duconseille, E., Falmagne, P. and Bernard, A. (1999) Cloning and characterization of A0EB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family. J. Biol. Chem. 274, 30451-30458
11. Baier, M. and Dietz, K. J. (1997) The plant 2-Cys peroxiredoxin BAS1 is a nuclear-encoded chloroplast protein, its expressional regulation, phylogenetic origin, and implications for its specific physiological function in plants. Plant J. 12, 179-190
12. Jin, D. Y., Chae, H. Z., Rhee, S. G. and Jeang, K. T. (1997) Regulatory role for a novel human thioredoxin-peroxidase in NF κB activation. J. Biol. Chem. 272, 30952-30961
13. Hoang, V. M., Foulk, R., Clauser, K., Burlingame, A., Gibson, B. W. and Fisher, S. J. (2001) Functional proteomics: examining the effects of hypoxia on the cytotrophoblast protein repertoire. Biochemistry 40, 4077-4086
14. Phelan, S. A., Johnson, K. A., Beier, D. R. and Paigen, B. (1998) Characterization of the murine gene encoding Aop2 (antioxidant protein 2) and identification of two highly related genes. Genomics 54, 132-139
15. Prosperi, M. T., Ferbus, D., Karczinski, I. and Goubin, G. (1993) A human cDNA corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. J. Biol. Chem. 268, 11050-11056
16. Rabilloud, T., Berthier, R., Vinçon, M., Ferbus, D., Goubin, G. and Lawrence, J. J. (1995) Early events in erythroid differentiation: accumulation of the acidic peroxidoxin (PRP/TSA/NKEF-B). Biochem. J. 312, 699-705
17. Rabilloud, T., Heller, M., Rigobello, M. P., Bindoli, A., Aebersold, R. and Lunardi, J. (2001) The mitochondrial antioxidant defence system and its response to oxidative stress. Proteomics 1, 1105-1110
18. Watabe, S., Kohno, H., Kouyama, H., Hiroi, T., Yago, N. and Nakazawa, T. (1994) Purification and characterization of a substrate protein for mitochondrial ATP-dependent protease in bovine adrenal cortex. J. Biochem. (Tokyo) 115, 648-654
19. Rabilloud, T., Heller, M., Gasnier, F., Luche, S., Rey, C., Aebersold, R., Benahmed, M., Louisot, P. and Lunardi, J. (2002) Proteomics analysis of cellular response to oxidative stress: evidence for in vivo over-oxidation of peroxiredoxins at their active site. J. Biol. Chem. 277, 19396-19401
20. Görg, A., Boguth, G., Obermaier, C. and Weiss, W. (1998) Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient. Electrophoresis 19, 1516-1519
21. Righetti, P. G. (1990) Immobilized pH Gradients. Theory and Methodology, Elsevier, Amsterdam
22. Rabilloud, T., Adessi, C., Giraudel, A. and Lunardi, J. (1997) Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 18, 307-316
23. Görg, A., Postel, W., Weser, J., Günther, S., Strahler, S. R., Hanash, S. M. and Somerlot, L. (1987) Elimination of point streaking on silver stained two-dimensional gels by addition of iodoacetamide to the equilibration buffer. Electrophoresis 8, 122-124
24. Sinha, P., Poland, J., Schnolzer, M. and Rabilloud, T. (2001) A new silver staining apparatus and procedure for matrix-assisted laser desorption/ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1, 835-840
25. Neuhoff, V., Arold, N., Taube, D. and Ehrhardt, W. (1988) Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255-262
26. Rabilloud, T., Strub, J. M., Luche, S., Van Dorsselaer, A. and Lunardi, J. (2001) A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels. Proteomics 1, 699-704
27. Gorman, J. J., Ferguson, B. L. and Nguyen, T. B. (1996) Use of 2,6-dihydroxyacetophenone for analysis of fragile peptides, disulphide bonding and small proteins by matrix-assisted laser desorption/ionization. Rapid Commun. Mass Spectrom. 10, 529-536
28. Drapeau, G. R. (1980) Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi. J. Biol. Chem. 255, 839-840
29. Mitsumoto, A., Takanezawa, Y., Okawa, K., Iwamatsu, A. and Nakagawa, Y. (2001) Variants of peroxiredoxins expression in response to hydroperoxide stress. Free Radical Biol. Med. 30, 625-635
30. Kim, J. R., Yoon, H. W., Kwon, K. S., Lee, S. R. and Rhee, S. G. (2000) Identification of proteins containing cysteine residues that are sensitive to oxidation by hydrogen peroxide at neutral pH. Anal. Biochem. 283, 214-221
31. Rabilloud, T., Kieffer, S., Procaccio, V., Louwagie, M., Courchesne, P. L., Patterson, S. D., Martinez, P., Garin, J. and Lunardi, J. (1998) Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome. Electrophoresis 19, 1006-1014