A novel monoclonal antibody screening method using the Luminex-100™ microsphere system

Journal of Immunological Methods

Volumn 267, Issue 2, 2002, Pages 165-171

  Seideman, Jonathan ^a   Peritt, David ^a  

^a Department of Immunobiology ^*  (United States)

Author keywords

Cytokine; Flow cytometry; IL 6; IL 6 receptor; Luminex; Neutralizing antibody

Indexed keywords

BIOTIN; CYTOKINE ANTIBODY; EPITOPE; INTERLEUKIN RECEPTOR; MICROSPHERE; MONOCLONAL ANTIBODY; NEUTRALIZING ANTIBODY; RECOMBINANT INTERLEUKIN 6;

ACCURACY; ANTIBODY SCREENING; ARTICLE; CARBOXYLATION; HUMAN; MOLECULAR PROBE; PRIORITY JOURNAL; RECEPTOR BINDING; SOLUBILITY;

ANTIBODIES, MONOCLONAL; BINDING, COMPETITIVE; BIOTIN; FLOW CYTOMETRY; HUMANS; INTERLEUKIN-6; KINETICS; LUMINESCENT MEASUREMENTS; MICROSPHERES; NEUTRALIZATION TESTS; RECEPTORS, INTERLEUKIN-6; RECOMBINANT PROTEINS;

EID: 0037105975     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00168-0     Document Type: Article

References (6)

1. Structure-function analysis of human IL-6. Epitope mapping of neutralizing monoclonal antibodies with amino- and carboxyl-terminal deletion mutants
2. Application of the luminex LabMAP in rapid screening for mutations in the cystic fibrosis transmembrane conductance regulator gene: A pilot study
3. IL-6 type cytokine receptor complexes: Hexamer, tetramer or both?
4. Multiplexed analysis of human cytokines by use of the FlowMetrix system
5. Multiple cytokines inhibit interleukin-6-dependent murine hybridoma/plasmacytoma proliferation
6. Multiplexed particle-based flow cytometric assays