Assessment of oxidative base damage to isolated and cellular DNA by HPLC-MS/MS measurement

Free Radical Biology and Medicine

Volumn 33, Issue 4, 2002, Pages 441-449

  Cadet, Jean ^a   Douki, Thierry ^a   Frelon, Sandrine ^a   Sauvaigo, Sylvie ^a   Pouget, Jean Pierre ^a   Ravanat, Jean Luc ^a  

^a Dept Rech Fond Matiere Cond   (France)

Author keywords

DNA oxidation; Free radicals; Liquid chromatograpghy tandem mass spectrometry; Oxidized nucleosides; Radical reactions

Indexed keywords

5 FORMYLDEOXYURIDINE; 5 HYDROXYDEOXYURIDINE; 5 HYDROXYMETHYLDEOXYURIDINE; 5,6 DIHYDRO 5,6 DIHYDROXYTHYMIDINE; 8 HYDROXYDEOXYGUANOSINE; 8 OXO 7,8 DIHYDRO 2' DEOXYADENOSINE; CELL DNA; DNA GLYCOSYLTRANSFERASE; NUCLEIC ACID BASE; NUCLEOSIDE DERIVATIVE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG; DNA; NUCLEOSIDE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

ACCURACY; COMET ASSAY; DIASTEREOISOMER; DNA DAMAGE; DNA EXTRACTION; DNA REPAIR; ELUTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; OXIDATION; PRIORITY JOURNAL; REVIEW; TANDEM MASS SPECTROMETRY; ULTRAVIOLET A RADIATION; ARTIFACT; CHEMISTRY; COMPARATIVE STUDY; DNA ADDUCT; ELECTROSPRAY MASS SPECTROMETRY; ISOLATION AND PURIFICATION; OXIDATION REDUCTION REACTION; PH; ULTRAFILTRATION;

ARTIFACTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COMET ASSAY; DNA; DNA ADDUCTS; DNA DAMAGE; DNA LIGASES; HYDROGEN-ION CONCENTRATION; NUCLEOSIDES; OXIDATION-REDUCTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; ULTRAFILTRATION;

EID: 0037101877     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(02)00820-1     Document Type: Article

References (82)

1. Chemical determination of free radical-induced damage to DNA
2. Reactions of oxyl radicals with DNA
3. Oxidative damage to DNA: Formation, measurement and biological significance
4. Hydroxyl radicals and DNA base damage
5. Lipid peroxidation-DNA damage by malondialdehyde
6. Indirect mutagenesis by oxidative DNA damage: Formation of the pyrimidopurinone adduct of deoxyguanosine by base propenal
7. Purine DNA adducts of 4,5-dioxovaleric acid and 2,4-decadienal
8. Reaction of cis- and trans-2-butene-1,4-dial with 2′-deoxycytidine to form stable oxadiazabicyclooctaimine adducts
9. Thymidine hydroperoxides: Structural assignment, conformational features, and thermal decomposition in water
10. Ionizing radiation induced decomposition of 2′-deoxycytidine in aerated solutions
11. 2′-Deoxycytidine glycols, a missing link in the free radical-mediated oxidation of DNA
12. Photosensitized oxidation of 5-methyl-2′-deoxycytidine by 2-methyl-1,4-naphthoquinone: Characterization of 5-hydroperoxymethyl-2′-deoxycytidine and stable methyl group oxidation products
13. 15N nuclear magnetic resonance analysis and chemical features of the two main radical oxidation products of 2′-deoxyguanosine: Oxazolone and imidazolone nucleosides
14. Photosensitized formation of 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-hydroxy-2′-deoxyguanosine) in DNA by riboflavin: A nonsinglet oxygen-mediated mechanism
15. Measurement of oxidative damage at pyrimidine bases in γ-irradiated DNA
16. Measurement of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-oxo-7,8-dihydroguanine in isolated DNA exposed to γ radiation in aqueous solution
17. Guanine radical cations are precursors of 7,8-dihydro-8-oxo-2′-deoxyguanosine but are not precursors of immediate strand breaks in DNA
18. Characterization and chemical stability of photo-oxidized oligonucleotides that contain 2,2-diamino-4-[(2-deoxy-β-D-erythro-pentofuranosyl)amino]-5(2H)- oxazolone
19. Modification of DNA bases by photosensitized one-electron oxidation
20. 8-Methoxydeoxyguanosine as an effective precursor of 2-aminoimidazolone, a major guanine oxidation product in one-electron oxidation of DNA
21. Possible cause of G-C → C-G transversion mutation by guanine oxidation product, imidazolone
22. Free radical-induced tandem base damage in DNA oligomers
23. Tandem base lesions are generated by hydroxyl radical within isolated DNA in aerated aqueous solution
24. Oxygen-dependent DNA damage amplification involving 5,6-dihydrothymidin-5-yl in a structural minimal system
25. Detecting DNA damage
26. 33P-postlabeling and electrochemical detection
27. Quantitative immunoanalysis of promutagenic 8-hydroxy-2′-deoxyguanosine in oxidized DNA
28. Quantitative immunohistochemical determination of 8-hydroxy-2′-deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model
29. Radioimmunoassay of a thymine glycol
30. Facts and artifacts in the measurement of oxidative base damage to DNA
31. 32P-postlabeling, immunological, and noninvasive assays
32. Mapping oxidative DNA damage using ligation-mediated polymerase chain reaction technology
33. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay
34. In vitro DNA damage/alkaline elution assay for predicting carcinogenic potential
35. Measurement of oxidative DNA damage using the comet assay
36. Measuring oxidative DNA damage by alkaline elution
37. The comet assay: What can it really tell us?
38. Oxidative DNA damage: Endonuclease fingerprinting
39. Measurement of DNA base damage in cells exposed to low doses of γ radiation: Comparison between the HPLC-EC and the comet assays
40. Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and γ radiation
41. DNA damage induced in cells by γ and UVA radiation as measured by HPLC/GC-MS and HPLC-EC and comet assays
42. Singlet oxygen induces oxidation of cellular DNA
43. Singlet oxygen DNA damage products: Formation and measurement
44. Hydroxyl free radical adduct of deoxyguanosine: Sensitive detection and mechanism of formation
45. Formation of 8-hydroxyguanosine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair
46. High-performance liquid chromatography-electrochemical assay for monitoring the formation of 8-oxo-7,8-dihydroadenine and its related 2′-deoxyribonucleoside
47. Determination of 8-oxo-purines in DNA by HPLC using amperometric detection
48. Endogenous oxidative damage of deoxycytidine in DNA
49. Application of capillary gas chromatography-mass spectrometry to chemical characterization of radiation-induced base damage of DNA: Implications for assessing DNA repair processes
50. Gas chromatography-mass spectrometry of free radical-induced products of pyrimidines and purines in DNA
51. Quantitative determination of oxidative base damage in DNA by stable isotope-dilution mass spectrometry
52. Determination of 8-oxoguanine in DNA by gas chromatography-mass spectrometry and HPLC-electrochemical detection. Overestimation of the background level of the oxidized base by the gas chromatography-mass spectrometry assay
53. Observation and prevention of an artifactual formation of oxidized DNA bases and nucleosides in the GC-EIMS method
54. New development in biochemical mass spectrometry: Electrospray ionization
55. Analysis of modified deoxynucleosides by electrospray ionization mass spectrometry
56. Determination of 8-hydroxyguanosine in biological tissues by liquid chromatography/electrospray ionization-mass spectrometry/mass spectrometry
57. Isotope dilution high-performance liquid chromatography-electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in biological samples
58. Simultaneous measurement of 8-oxo-2′-deoxyguanosine and 8-oxo-2′-deoxyadenosine by HPLC-MS/MS
59. Measurement of 8-oxo-2′-deoxyguanosine and 8-oxo-2′-deoxyadenosine in DNA and human urine by high-performance liquid chromatography-electrospray tandem mass spectrometry
60. High-performance liquid chromatography-tandem mass spectrometry for the measurement of radiation-induced base damage to isolated and cellular DNA
61. Chemical ionization mass spectrometry of nucleosides
62. Comparison of negative and positive ion electrospray tandem mass spectrometry for the liquid chromatography tandem mass spectrometry analysis of oxidized deoxynucleosides
63. Measurement of 8-hydroxy-2′-deoxyguanosine in DNA by high-performance liquid chromatography-mass spectrometry: Comparison with measurement by gas chromatography-mass spectrometry
64. Identification and quantification of 8,5′-cyclo-2′-deoxy-adenosine in DNA by liquid chromatography-mass spectrometry
65. Measurement of 8-hydroxy-2′-deoxyadenosine in DNA by liquid chromatography-mass spectrometry
66. Measurement of the main photo-oxidation products of 2′-deoxyguanosine using chromatographic methods coupled to mass spectrometry
67. Inducible repair of thymine glycol detected by an ultrasensitive assay for DNA damage
68. Electrospray-mass spectrometry characterization and measurement of far-UV-induced thymine photoproducts
69. Formation of the main UV-induced thymine dimeric lesions within isolated and cellular DNA as measured by high-performance liquid chromatography-tandem mass spectrometry
70. Individual determination of the yield of the main UV-induced dimeric pyrimidine photoproducts in DNA suggests a high mutagenicity of CC photolesions
71. Ionizing radiation causes gretaer DNA base damage in radiation-sensitive mutant M10 cells than in parent mouse lymphoma L5178Y cells
72. Oxidative modification of DNA bases in rat liver and lung during chemical carcinogenesis and aging
73. Vitamin C exhibits pro-oxidant properties
74. Iron-induced DNA damage and its repair in primary rat hepatocyte culture
75. Progression of human breast cancers to the metastatic state is linked to hydroxyl radical-induced DNA damage
76. Measurement of oxidative DNA damage by gas chromatography-mass spectrometry: Ethanethiol prevents artifactual generation of oxidized DNA bases
77. Purification of genomic DNA from human whole blood by isopropanol-fractionation with concentrated NaI and SDS
78. A reliable assessment of 8-oxo-2′-deoxyguanosine levels in nuclear and mitochondrial DNA using the sodium iodide method to isolate DNA
79. Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue
80. DNA oxidation matters: The HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine
81. A novel HPLC procedure for the analysis of 8-oxoguanine in DNA