The splicing of U12-type introns can be a rate-limiting step in gene expression

EMBO Journal

Volumn 21, Issue 14, 2002, Pages 3804-3815

  Patel, Abhijit A ^a   McCarthy, Matthew ^a   Steitz, Joan A ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Pre mRNA; Regulation of gene expression; Spliceosome; Splicing rate; U12 type intron

Indexed keywords

MESSENGER RNA PRECURSOR;

ANIMAL CELL; ARTICLE; DROSOPHILA MELANOGASTER; GENE EXPRESSION; GENE EXPRESSION REGULATION; INTRON; MUTATION; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; SPLICEOSOME; TRANSCRIPTION REGULATION;

ANIMALS; BASE SEQUENCE; CELLS, CULTURED; DNA; DROSOPHILA; GENE EXPRESSION REGULATION; INTRONS; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA SPLICING; SODIUM-HYDROGEN ANTIPORTER;

ANIMALIA; ARACHNIDA; DROSOPHILA MELANOGASTER; HEXAPODA; MELANOGASTER; METAZOA;

EID: 0037099491     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf297     Document Type: Article

References (36)

1. Identification of a regulated pathway for nuclear pre-mRNA turnover
2. Expression of two Xenopus laevis ribosomal protein genes in injected frog oocytes. A specific splicing block interferes with the L1 RNA maturation
3. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
4. Evolutionary fates and origins of U12-type introns
5. The accumulation of mature RNA for the Xenopus laevis ribosomal protein L1 is controlled at the level of splicing and turnover of the precursor RNA
6. Inefficient in vitro splicing of the regulatory intron of the L1 ribosomal protein gene of X.laevis depends on suboptimal splice site sequences
7. In vitro study of processing of the intron-encoded U16 small nucleolar RNA in Xenopus laevis
8. Terminal intron dinucleotide sequences do not distinguish between U2- and U12-dependent introns
9. Identification of the sequences responsible for the splicing phenotype of the regulatory intron of the L1 ribosomal protein gene of Xenopus laevis
10. Quantitative RT-PCR: Pitfalls and potential
11. Processing of the primary transcript for the rat growth hormone gene in vivo
12. Ordered splicing of thymidine kinase pre-mRNA during the S phase of the cell cycle
13. Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice sites
14. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns
15. RNA polymerase II and the integration of nuclear events
16. Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F
17. A reappraisal of non-consensus mRNA splice sites
18. Order of intron removal during splicing of endogenous adenine phosphoribosyltransferase and dihydrofolate reductase pre-mRNA
19. At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells
20. A computational scan for U12-dependent introns in the human genome sequence
21. Two-color GFP expression system for C.elegans
22. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
23. Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.
24. Splicing signals in Drosophila: Intron size, information content and consensus sequences
25. The divergent U12-type spliceosome is required for pre-mRNA splicing and is essential for development in Drosophila
26. Cloning, human chromosomal assignment and adipose and hepatic expression of the CL-6/INSIG1 gene
27. Expression of the gene for ribosomal protein L1 in Xenopus embryos: Alteration of gene dosage by microinjection
28. Exon definition may facilitate splice site selection in RNAs with multiple exons
29. Classification of introns: U2-type or U12-type
30. Trans splicing of mRNA precursors
31. A putative voltage-gated sodium channel α subunit (PpSCN1) from the hydrozoan jellyfish, Polyorchis penicillatus: Structural comparisons and evolutionary considerations
32. The small nuclear ribonucleoprotein E protein gene contains four introns and has upstream similarities to genes for ribosomal proteins
33. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
34. A novel spliceosome containing U11, U12 and U5 snRNPs excises a minor class (AT-AC) intron in vitro
35. The intranuclear site of excision of each intron in Balbiani ring 3 pre-mRNA is influenced by the time remaining to transcription termination and different excision efficiencies for the various introns
36. U1-mediated exon definition interactions between AT-AC and GT-AG introns