Identification of polysaccharide binding proteins by affinity electrophoresis in inhomogeneous polyacrylamide gels and subsequent SDS-PAGE/matrix-assisted laser desorption ionization-time of flight analysis

Analytical Biochemistry

Volumn 304, Issue 2, 2002, Pages 180-192

  Eckermann, Nora ^a   Fettke, Jörg ^a   Steup, Martin ^a  

^a UNIVERSITY OF POTSDAM   (Germany)

Author keywords

Affinity electrophoresis; Branching enzyme; Glucan phosphorylase; MALDI MS analysis of proteins; Polysaccharide binding proteins; Protein carbohydrate interactions; Tryptic digestion

Indexed keywords

DESORPTION; ELECTROPHORESIS; GELS; IONIZATION; LIGANDS; MASS SPECTROMETRY;

BINDING PROTEINS; BRANCHING ENZYME; GLUCAN PHOSPHORYLASE; MALDI-MS; PROTEIN-CARBOHYDRATE INTERACTIONS; TRYPTIC DIGESTION;

PROTEINS;

1,4 ALPHA GLUCAN BRANCHING ENZYME; CARBOHYDRATE BINDING PROTEIN; PHOSPHORYLASE;

ARTICLE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR INTERACTION; NONHUMAN; PHYTOCHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POTATO; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION;

EID: 0037092969     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5628     Document Type: Article

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