Retrieval of mRNA from paraffin-embedded human infant brain tissue for non-radioactive in situ hybridization using oligonucleotides

Journal of Neuroscience Methods

Volumn 115, Issue 2, 2002, Pages 129-136

  Relf, Bronwyn L ^a   Machaalani, Rita ^a   Waters, Karen A ^a,b  

^a UNIVERSITY OF SYDNEY   (Australia)

^b SYDNEY CHILDREN'S HOSPITAL   (Australia)

Author keywords

Brainstem; In situ hybridization; Infant; Microwave; mRNA; Non radioactive; Oligonucleotide

Indexed keywords

CITRIC ACID; EDETIC ACID; FORMALDEHYDE; MESSENGER RNA; OLIGONUCLEOTIDE; PARAFFIN;

ARTICLE; AUTOCLAVE; BRAIN TISSUE; CONTROLLED STUDY; DIGESTION; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IN SITU HYBRIDIZATION; INFANT; MEDICAL RESEARCH; MICROWAVE RADIATION; MORPHOLOGY; PRIORITY JOURNAL; TECHNIQUE;

AGED; BRAIN; BUFFERS; HUMANS; IMMUNOLOGIC TECHNIQUES; IN SITU HYBRIDIZATION; INFANT; MIDDLE AGED; OLIGONUCLEOTIDE PROBES; PARAFFIN EMBEDDING; RNA, MESSENGER;

EID: 0037091123     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00003-1     Document Type: Article

References (13)

1. Hippocampal loss of N-methyl-D-aspartate receptor subunit 1 mRNA in chronic temporal lobe epilepsy
2. Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes
3. Detection and quantification of hippocampal synaptophysin messenger RNA in schizophrenia using autoclaved, formalin-fixed, paraffin wax-embedded sections
4. Antigen retrieval in formaldehyde-fixed human brain tissue
5. Molecular cloning and chromosomal localization of the key subunit of the human N-methyl-D-aspartate receptor
6. A simple, reliable, and sensitive method for nonradioactive in situ hybridization: Use of microwave heating to improve hybridization efficiency and preserve tissue morphology
7. Antigen preservation in microwave-irradiated tissues: A comparison with formaldehyde fixation
8. The use of microwave irradiation as a pre-treatment to in situ hybridization for the detection of measles virus and chicken anaemia virus in formalin-fixed paraffin-embedded tissue
9. A comparison of digoxigenin and biotin labelled DNA and RNA probes for in situ hybridization
10. Quantitative comparison of pre-treatment regimens used to sensitize in situ hybridization using oligonucleotide probes on paraffin-embedded brain tissue
11. Retrieval of cellular mRNA in paraffin-embedded human brain using hydrated autoclaving
12. Molecular cloning, functional expression, and pharmacological characterization of an N-methyl-D-aspartate receptor subunit from human brain
13. Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies