A simple, reliable, and sensitive method for nonradioactive in situ hybridization: Use of microwave heating to improve hybridization efficiency and preserve tissue morphology

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 3, 1996, Pages 281-287

  Lan, Hui Y ^a   Mu, Wei ^a   Ng, Yee Y ^a   Nikolic Paterson, David J ^a   Atkins, Robert C ^a  

^a MONASH MEDICAL CENTRE   (Australia)

Author keywords

Digoxigenin; In situ hybridization; Microwave; Tissue digestion; Tissue morphology

Indexed keywords

DIGOXIGENIN; MESSENGER RNA; PARAFORMALDEHYDE; PROTEINASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IN SITU HYBRIDIZATION; MICROSCOPY; MICROWAVE COOKING; MICROWAVE OVEN; MORPHOLOGY; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0029968518     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.3.8648089     Document Type: Article

References (13)

1. Shi S-R, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemcal staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39-741
2. Shi S-R, Chaiwun B, Young L, Cote RJ, Taylor CR. Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed, paraffin sections. J Histochem Cytochem 1993;41:1599
3. Lan HY, Mu W, Nikcolic-Paterson DJ, Atkins RC A novel, simple, reliable, and sensitive method for multiple immunoenzyme staining: use of microwave oven heating to block antibody crossreactivity and retrieve antigens. J Histochem Cytochem 1995;43:97
4. Henke R-P, Ayhan N. Enhancement of hybridization efficiency in interphase cytogenetics on paraffin-embedded tissue sections by microwave treatment Anal Cell Pathol 1994;6:319
5. Lan HY, Nikolic-Paterson DJ, Jarama M, Vannice JL, Atkins RC. Suppression of experimental crescentic glomerulonephritis by the interleukin-1 receptor antagonist. Kidney Int 1993;43:479
6. Williams AJ, Atkins RC, Fries JWU, Gimbrone MA Jr, Cybulsky MI, Colins T. Nucleotide sequence of rat vascular cell adhesion molecule-1 cDNA. Biochim Biophys Acta 1992;1131:214
7. Mitchell R, Bacher M, Bernhagen J, Pushkarskaya T, Seldin MF, Bucala R. Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF). J Immunol 1995;154:3863
8. Swift GH, Dagorn J-C, Ashley PL, Cummings SW, MacDonald RJ. Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme Proc Natl Acad Sci USA 1982;79:7263
9. Yamawaki M, Zurbriggen A, Richard A, Vandevelde M. Saponin treatment for in situ hybridization maintains good morphological preservation. J Histochem Cytochem 1993;41:105
10. Bernhagen J, Calandra T, Mitchell RA, Martin SB, Tracey KJ, Voelter W, Manogue KR, Cerami A, Bucala R. MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature 1993;365:756
11. Wilcox JN. Fundamental principles of in situ hybridization. J Histochem Cytochem 1993;41:1725
12. Lan HY, Nikolic-Paterson DJ, Atkins RC. Local macrophage proliferation in experimental Goodpasture's syndrome Nephrology 1995;1:151
13. Lan HY, Nikolic-Paterson DJ, Mu W, Atkins RC. Local macrophage proliferation in multinucleated giant cell and granuloma formation in experimental Goodpasture's syndrome Am J Pathol, in press