Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties

Genes and Development

Volumn 16, Issue 6, 2002, Pages 693-706

  Gudjonsson, Thorarinn ^a   Villadsen, René ^a   Nielsen, Helga Lind ^a,b   Rønnov Jessen, Lone ^b   Bissell, Mina J ^c   Petersen, Ole William ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Breast; Human cell; Immortal; K19; Stem cell

Indexed keywords

CLAUDIN 1; EPITHELIAL MEMBRANE ANTIGEN; KERATIN; OCCLUDIN; SIALOMUCIN;

ARTICLE; BREAST EPITHELIUM; BREAST RECONSTRUCTION; CELL COMPARTMENTALIZATION; CELL FUNCTION; CELL IMMORTALIZATION; CELL ISOLATION; CELL LINE; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; NUDE MOUSE; PRIORITY JOURNAL; STEM CELL; SUBCUTANEOUS TISSUE; WART VIRUS;

ACTINS; ANIMALS; BREAST; CELL LINE; COLLAGEN; DRUG COMBINATIONS; EPITHELIAL CELLS; HUMANS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; KERATINS; LAMININ; MEMBRANE PROTEINS; MICE; MICE, INBRED BALB C; MICE, NUDE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PHENOTYPE; PROTEOGLYCANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS;

HUMAN PAPILLOMAVIRUS TYPES; MUS MUSCULUS; PAPILLOMAVIRUS;

EID: 0037087738     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.952602     Document Type: Article

References (50)

1. The development of epithelial phenotypes in the human fetal and infant breast
2. The biology of the 17-1A antigen (Ep-CAM)
3. Preneoplastic transformation of human mammary epithelial cells
4. The role of retinoblastoma and p53 tumor suppressor pathways in human mammary epithelial cell immortalization
5. Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements
6. Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours
7. Tissue structure, nuclear organization, and gene expression in normal and malignant breast
8. Cell differentiation by extracellular matrix components
9. Cytokeratins and cell differentiation in the pancreas
10. Switch in the expression of the K19/K18 keratin genes as a very early evidence of testicular differentiation in the rat
11. Long-term culture and immortalization of epithelial cells from normal adult human pancreatic ducts transfected by the E6E7 gene of human papilloma virus 16
12. Viral oncogenes accelerate conversion to immortality of cultured conditionally immortal human mammary epithelial cells
13. Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition
14. The E6 and E7 genes of human papillomavirus type 6 have weak immortalizing activity in human epithelial cells
15. Proliferative heterogeneity in the human prostate: Evidence for epithelial stem cells
16. Epithelial cell differentiation pathways in the human prostate: Identification of intermediate phenotypes by keratin expression
17. A collagen substrate enhances the sealing capacity of tight junctions of A6 cell monolayers
18. Specific association of human telomerase activity with immortal cells and cancer
19. Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: Keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage
20. Characterization of epithelial phenotypes in mortal and immortal human breast cells
21. Human mammary luminal epithelial cells contain progenitors to myoepithelial cells
22. Characterization of epithelial membrane antigen expression in human mammary epithelium by ultrastructural immunoperoxidase cytochemistry
23. Preservation of defined phenotypic traits in short-time cultured human breast carcinoma derived epithelial cells
24. Growth factor control of myoepithelial-cell differentiation in cultures of human mammary gland
25. Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells
26. Differentiation and cancer in the mammary gland: Shedding new light on an old dichotomy
27. In vitro transformation of cell lines from human salivary gland tumors
28. Stem cells and the development of mammary cancers in experimental rats and humans
29. The origin of the myofibroblasts in breast cancer: Recapitulation of tumor environment in culture unravels diversity and implicates converted fibroblasts and recruited smooth muscle cells
30. Cellular changes involved in conversion of normal to malignant breast: The importance of the stromal reaction
31. Malignant MCF10CA1 cell lines derived from premalignant human breast epithelial MCF10AT cells
32. Evaluation of proliferating cell types in human and mouse mammary gland by a double immunostaining procedure
33. Clonal characterization of mouse mammary luminal epithelial and myoepithelial cells separated by fluorescence-activated cell sorting
34. Differentiation of separated mouse mammary luminal epithelial and myoepithelial cells cultured on EHS matrix analyzed by indirect immunofluorescence of cytoskeletal antigens
35. Experimental mammary epithelial morphogenesis in an in vivo model: Evidence for distinct cellular progenitors of the ductal and lobular phenotype
36. A morphologically distinct candidate for an epithelial stem cell in mouse mammary gland
37. Mammary epithelial stem cells
38. E7-transduced human breast epithelial cells show partial differentiation in three-dimensional culture
39. Culture systems for study of human mammary epithelial cell proliferation, differentiation, and transformation
40. Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor beta growth inhibition in p16INK4A(-) human mammary epithelial cells
41. Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast
42. Characterization of bipotent mammary epithelial progenitor cells in normal adult human breast tissue
43. Expression of cytokeratin 19 during human liver organogenesis
44. Cytokeratins 8 and 19 in the mouse placental development
45. Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: Relation to in vivo phenotypes ad influence of medium
46. Immortalization of distinct human mammary epithelial cell types by human papilloma virus 16 E6 or E7
47. An atlas of subgross pathology of the human breast with special reference to possible precancerous lesions
48. Immortalization of normal human bronchial epithelial cells by human papillomaviruses 16 or 18
49. Phenotypic characterization of collagen gel embedded primary human breast epithelial cells in athymic nude mice
50. Cell transformation by the E7 oncoprotein of human papillomavirus type 16: Interactions with nuclear and cytoplasmic target proteins