Rab5a GTPase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils

Journal of Cell Science

Volumn 115, Issue 6, 2002, Pages 1321-1330

  Perskvist, Nasrin ^a   Roberg, Karin ^a   Kulyté, Agné ^a   Stendahl, Olle ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

Author keywords

Fusion; Mycobacterium tuberculosis; Neutrophil; Phagocytosis; Rab5

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; GUANOSINE TRIPHOSPHATASE; LACTOFERRIN; MEMBRANE PROTEIN; RAB PROTEIN; RAB5A PROTEIN; SNARE PROTEIN; SYNTAXIN; UNCLASSIFIED DRUG;

ARTICLE; BIOGENESIS; CELL FUSION; CELL ORGANELLE; CELL VACUOLE; COMPLEX FORMATION; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; ENDOCYTOSIS; ENDOSOME; HUMAN; HUMAN CELL; INTERNALIZATION; LYSOSOME; MOLECULAR BIOLOGY; MYCOBACTERIUM TUBERCULOSIS; NEUTROPHIL; PHAGOCYTOSIS; PHAGOSOME; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; REGULATORY MECHANISM; STAPHYLOCOCCUS AUREUS; WESTERN BLOTTING;

CELLS, CULTURED; CYTOPLASM; DOWN-REGULATION; ENDOSOMES; HUMANS; MEMBRANE FUSION; MEMBRANE PROTEINS; NEUTROPHILS; ORGANELLES; PHAGOSOMES; QA-SNARE PROTEINS; RAB5 GTP-BINDING PROTEINS;

CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0037087512     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (53)

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2. Phagocytosed live monocytogenes Listeria influences Rab5-regulated in vitro phagosome-endosome fusion
3. Distinct granule population in human neutrophils and lysosomal organelles identified by immuno-electron microscopy
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8. Cooperative regulation of endocytosis by three Rab5 isoforms
9. Isolation of mononuclear cells and granulocytes from human blood
10. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins
11. Isolation and characterization of pathogen-containing phagosomes
12. Azurophilic granules of human neutrophilic leukocytes are deficient in lysosme-associated membrane proteins but retain the mannose 6-phosphate recognition marker
13. Deviant expression of Rab5 on phagosomes containing the intracellular pathogens Mycobacterium tuberculosis and Legionella pneumophila is associated with altered phagosomal fate
14. Transfer of phagocytosed particles to the parasitophorous vacuole of Leishmania mexicana is a transient phenomenon preceding the acquisition of annexin I by the phagosome
15. rab8 in retinal photoreceptors may participate in rhodopsin transport and in rod outer segment disk morphogenesis
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17. Localization of five annexins in J774 macrophages and on isolated phagosomes
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19. Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest
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23. Cytosolic free calcium elevation mediates the phagosome-lysosome fusion during phagocytosis in human neutrophils
24. The opsonizing ligand on Salmonella typhimurium influences incorporation of specific, but not azurophil granule constituents into neutrophil phagosomes
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29. t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase
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31. Neisseria gonorrhoeae porin modulates phagosome maturation
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33. Live Salmonella recruits N-ethylmaleimide-sensitive fusion protein on phagosomal membrane and promotes fusion with early endosome
34. SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes
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37. High resolution two-dimentional electrophoresis
38. Activation of human neutrophils by Mycobacterium tuberculosis H37Ra involves phospholipase C gamma 2, Shc adapter protein, and p38 mitogen-activated protein kinase
39. Vac1p coordinates Rab and hosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
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41. Assay of phagocytic cell functions
42. Dynamics of rab5 activation in endocytosis and phagocytosis
43. Mycobacterium avium- and Mycobacterium tuberculosis-containing vacuoles are dynamic, fusion-competent vesicles that are accessible to glycosphingolipids from the host cell plasmalemma
44. Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils
45. The Rab5 effector EEA1 interacts directly with syntaxin-6
46. 2+ stores during phagocytosis in human neutrophils
47. Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase
48. Mycobacterium-containing phagosomes are accessible to early endosomes and reflect a transitional state in normal phagosome biogenesis
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50. Arrest of mycobacterial phagosome maturation is caused by a block in vesicle fusion between stages controlled by rab5 and rab7
51. Sucellular localization of the small GTPase Rab5a in resting and stimulated human neutrophils
52. Killing of phagocytosed Staphylococcus aureus by human neutrophils requires intracellular free calcium
53. Phagosome-lysosome fusion is a calcium-independent event in macrophages