Efficient PCR-based gene disruption in Saccharomyces strains using intergenic primers

Yeast

Volumn 19, Issue 4, 2002, Pages 319-328

  Reid, Robert J D ^a   Sunjevaric, Ivana ^a   Kedacche, Mehdi ^a,b   Rothstein, Rodney ^a  

^a Columbia University College of Physicians and Surgeons ^*  (United States)

^b CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

Author keywords

Deletion consortium; Gene distruption; Saccharomyces cerevisiae; Yeast

Indexed keywords

BIOCHEMICAL MARKER; KANAMYCIN; PRIMER DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; FUNGAL STRAIN; GENE AMPLIFICATION; GENE DISRUPTION; GENE FUNCTION; GENE LIBRARY; GENE TRANSFER; GENETIC TRANSFORMATION; KLUYVEROMYCES; METHODOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE TAGGED SITE;

DNA PRIMERS; GENE DELETION; KLUYVEROMYCES; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

KLUYVEROMYCES; KLUYVEROMYCES LACTIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037087284     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.817     Document Type: Article

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