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Comparison of the intensities of selected infrared bands of the monolayer referenced to those of bulk sample allows us to estimate the twist and tilt angle of the molecules forming the monolayer (see eqs 5-7 in ref 14a). The values obtained are 60° and 15° for twist and tilt angles, respectively. The values of twist and tilt angles were similar for SAM 2 and SAM 4. These results should be, however, treated only as approximation, because direct comparison of amide II bands for monolayer and bulk sample is difficult due to the fact that intensities of these bands are strongly affected by the extent of hydrogen bonding. The latter may be different within the monolayer and the bulk phase sample.
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