A revised mechanism for the inactivation of bovine liver enoyl-CoA hydratase by (methylenecyclopropyl)formyl-CoA based on unexpected results with the C114A mutant

Biochemistry

Volumn 41, Issue 6, 2002, Pages 1843-1852

  Agnihotri, Gautam ^a,b,c   He, Shouming ^d   Hong, Lin ^a,b   Dakoji, Srikanth ^a,b,e   Withers, Stephen G ^d   Liu, Hung Wen ^a,b  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b University of Minnesota   (United States)

^c GLAXOSMITHKLINE   (United States)

^d UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RING CLEAVAGE;

CATALYSTS; ENZYMES; REACTION KINETICS;

BIOCHEMISTRY;

(METHYLENECYCLOPROPYL)FORMYL COENZYME A; COENZYME A; ENOYL COENZYME A HYDRATASE; ENZYME INHIBITOR; LIVER ENZYME; PEPSIN A; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATTLE; ENZYME INACTIVATION; ENZYME KINETICS; ESCHERICHIA COLI; LIVER; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ACYL COENZYME A; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BASE SEQUENCE; CATTLE; DNA; ENOYL-COA HYDRATASE; ENZYME INHIBITORS; KINETICS; LIVER; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

BOS TAURUS; BOVINAE; ESCHERICHIA COLI;

EID: 0037065701     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0119363     Document Type: Article

References (36)