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27
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0011948655
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note
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2 was 1.38 mM. For the nonenzymatic chlorination, 0.4 mM TMB or DMP were treated in 50 mL of 0.12 M citrate phosphate buffer, pH 3 with 3 mL of 0.353 M NaOCl added by syringe pump over a 100-min interval. The total added NaOCl was 20 mM. The solution was stirred overnight before extraction. Each experiment was performed in triplicate. In the CPO-catalyzed reactions, the KCl was the reagent in excess, and in the noncatalyzed reaction, the NaOCl was the reagent in excess. The chlorinated compounds were extracted into pentane and analyzed by GC-MS.
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28
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0011947576
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note
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37Cl of the sample.
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30
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0011976724
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note
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- was then analyzed as described in ref 26.
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32
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