Recombinant lysine:N6-hydroxylase: Effect of cysteine→alanine replacements on structural integrity and catalytic competence

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1594, Issue 2, 2002, Pages 219-233

  Dick, Scott ^a   Siemann, Stefan ^a   Frey, Harold E ^a   Lepock, James R ^a   Viswanatha, Thammaiah ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Cysteine modification; Flavoprotein; Lysine mono oxygenase; Mutagenesis; N6 Hydroxylysine

Indexed keywords

2,6 DICHLOROPHENOLINDOPHENOL; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); ALANINE; CYSTEINE; IODOACETIC ACID; LYSINE:N 6 HYDROXYLASE; OXYGENASE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BIOLOGICAL MONITORING; CATALYSIS; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; THERMOSTABILITY;

ALANINE; CALORIMETRY, DIFFERENTIAL SCANNING; CYSTEINE; DITHIONITROBENZOIC ACID; ENZYME STABILITY; FLAVIN-ADENINE DINUCLEOTIDE; HEAT; IOPANOIC ACID; KINETICS; MIXED FUNCTION OXYGENASES; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 0037060469     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(01)00305-3     Document Type: Article

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