Studies with lysine N6-hydroxylase. Effect of a mutation in the assumed FAD binding site on coenzyme affinities and on lysine hydroxylating activity

Biological Chemistry

Volumn 380, Issue 1, 1999, Pages 47-54

  Stehr, Matthias ^a   Smau, Liliana ^b   Singh, Mahavir ^c   Seth, Oliver ^b   Macheroux, Peter ^d   Ghisla, Sandro ^b   Diekmann, Hans ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b UNIVERSITY OF KONSTANZ   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^d ETH ZURICH   (Switzerland)

Author keywords

Aerobactin; Enzyme kinetics; Flavoprotein in monooxygenase; Lysine; Nucleotide binding; Site directed mutagenesis

Indexed keywords

FLAVINE ADENINE NUCLEOTIDE; LYSINE; MUTANT PROTEIN; PROCOLLAGEN LYSINE 2 OXOGLUTARATE 5 DIOXYGENASE; PROLINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; GLYCINE; LYSINE N EPSILON HYDROXYLASE; LYSINE N-EPSILON HYDROXYLASE; MIXED FUNCTION OXIDASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING SITE; COENZYME; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROXYLATION; NONHUMAN; PH; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; BIOSYNTHESIS; COMPARATIVE STUDY; GENE VECTOR; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; SYNTHESIS;

ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; FLAVIN-ADENINE DINUCLEOTIDE; GENETIC VECTORS; GLYCINE; HYDROXYLATION; KINETICS; LYSINE; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROLINE; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032586828     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.1999.006     Document Type: Article

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