Identification of the catalytic residues of α-amino acid ester hydrolase from Acetobacter turbidans by labeling and site-directed mutagenesis

Journal of Biological Chemistry

Volumn 277, Issue 32, 2002, Pages 28474-28482

  Polderman Tijmes, Jolanda J ^a   Jekel, Peter A ^a   Jeronimus Stratingh, C Margot ^b   Bruins, Andries P ^b   Van Der Laan, Jan Metske ^c   Sonke, Theo ^d   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c DSM FOOD SPECIALTIES   (Netherlands)

^d DSM RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; CATALYSIS; DATABASE SYSTEMS; ENZYMES; ESTERS; HYDROLYSIS; MASS SPECTROMETRY; MUTAGENESIS; SYNTHESIS (CHEMICAL);

ACETOBACTER TURBIDANCE; CATALYTIC RESIDUES; ELECTROSPRAY MASS SPECTROMETRY; SERINE CONSENSUS SEQUENCE;

AMINO ACIDS;

ALPHA AMINO ACID ESTER HYDROLASE; ASPARTIC ACID; BACTERIAL ENZYME; BETA LACTAMASE; HISTIDINE; SERINE; TYROSINE; UNCLASSIFIED DRUG; ALPHA AMINO ACID ESTERASE; ALPHA-AMINO ACID ESTERASE; ANTIINFECTIVE AGENT; CARBOXYLESTERASE; LACTAM; METHIONINE; PEPTIDE; THREONINE;

ACETOBACTER; ACETOBACTER TURBIDANS; ARTICLE; BACTERIAL STRAIN; CATALYSIS; CELL LABELING; CHEMICAL BOND; CIRCULAR DICHROISM; CONTROLLED STUDY; COVALENT BOND; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN HYDROLYSIS; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL MODEL; CHEMISTRY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; SEQUENCE HOMOLOGY; TIME;

ACETOBACTER PASTEURIANUS;

ACETOBACTER; AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CATALYSIS; CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; CLONING, MOLECULAR; CONSERVED SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; KINETICS; LACTAMS; METHIONINE; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; THREONINE; TIME FACTORS;

EID: 0037047357     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M204143200     Document Type: Article

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