Downregulation of PGY1/MDR1 mRNA level in human KB cells by antisense oligonucleotide conjugates. RNA accessibility in vitro and intracellular antisense activity

Biochimica et Biophysica Acta - Gene Structure and Expression

Volumn 1576, Issue 1-2, 2002, Pages 143-147

  Kostenko, Elena V ^a   Laktionov, Pavel P ^a   Vlassov, Valentin V ^a   Zenkova, Marina A ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

Author keywords

Antisense oligonucleotide conjugate; KB cell; Multiple drug resistance; PGY1 MDR1 mRNA

Indexed keywords

5' BISPYRENYL 3' AMINOHEXYLOLIGODEOXYRIBONUCLEOTIDE; ANTISENSE OLIGONUCLEOTIDE; MESSENGER RNA; MULTIDRUG RESISTANCE PROTEIN 1; NUCLEASE; RNA; UNCLASSIFIED DRUG;

ARTICLE; CELL STRAIN KB; CONJUGATE; CONTROLLED STUDY; GENE ACTIVITY; GENE EXPRESSION; GENE OVEREXPRESSION; GENE TARGETING; GENETIC ANALYSIS; HUMAN; HUMAN CELL; MULTIDRUG RESISTANCE; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

DOWN-REGULATION; HUMANS; MOLECULAR STRUCTURE; OLIGONUCLEOTIDES, ANTISENSE; P-GLYCOPROTEIN; PHENOTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR CELLS, CULTURED;

EID: 0037036152     PISSN: 01674781     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4781(02)00339-1     Document Type: Article

References (23)

1. Expression of multidrug resistance gene in human cancers
2. The MDR1 gene responsible for multidrug resistance codes for P-glycoprotein
3. Oligonucleotide modulation of multidrug resistance
4. Concerning inhibition of multiple drug resistance genes
5. Overcoming multidrug resistance in human tumour cells using free and liposomally encapsulated antisense oligodeoxynucleotides
6. Modulation of drug resistance in a daunorubicine resistant subline with oligonucleotide methyl-phosphonates
7. Modulation of multidrug resistance in human leukaemia cells with MDR1-targeted antisense oligonucleotides using variable treatment schedules
8. Inhibition of expression of the multidrug resistance-associated P-glycoprotein by phosphorothioate and 5′ cholesterol-conjugated phosphorothioate antisense oligonucleotides
9. Modulation of multidrug resistance gene (mdr-1) with antisense oligodeoxynucleotides
10. Potent antisense oligonucleotides to the human multidrug resistance-1 mRNA are rationally selected by mapping RNA-accessible sited with oligonucleotide libraries
11. Inhibition of P-glycoprotein and recovery of drug sensitivity of human acute leukaemic blast cells by multidrug resistance gene (MDR1) antisense oligonucleotides
12. Effects of RNA secondary structure on cellular antisense activity
13. Secondary structure of the 5′-region of PGY1/MDR1 mRNA
14. 5′-Bis-pyrenylated oligonucleotides displaying excimer fluorescence provide sensitive probes of RNA sequence and structure
15. 5′-Bis-pyrenylated oligonucleotides display enhanced excimer fluorescence upon hybridization with DNA and RNA
16. Sensitised photomodification by binary systems: I. Synthesis of oligonucleotide reagents, and the effect of their structure on the efficacy of target modification
17. Inexpensive SDS/phenol method for RNA extraction from tissues
18. Evaluation of a tetrazolium-based semiautomatic colorimetric assay: Assessment of chemosensitivity testing
19. Quantitative analysis of MDR1 multidrug resistance gene expression in human tumors by polymerase chain reaction
20. Monitoring mRNA expression by polymerase chain reaction: The "primer-dropping" method
21. Standardization of mRNA titration using a polymerase chain reaction method involving co-amplification with a multispecific internal control