Exocytosis studies in a chromaffin cell-free system: Imaging of single-vesicle exocytosis in a chromaffin cell-free system using total internal reflection fluorescence microscopy

Annals of the New York Academy of Sciences

Volumn 971, Issue , 2002, Pages 257-261

  Wiegand, Ulrich K ^a   Don Wauchope, Andrew ^a   Matskevich, Ioulia ^a   Duncan, Rory R ^a   Greaves, Jennifer ^a   Shipston, Michael J ^a   Apps, David K ^a   Chow, Robert H ^b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

2D+1 image analysis; Acridine orange; Chromaffin cell free system; EGFP; Single vesicle exocytosis; Total internal reflection fluorescence microscopy

Indexed keywords

ACRIDINE ORANGE; CALCIUM;

ANIMAL CELL; CELL FREE SYSTEM; CELL GRANULE; CHROMAFFIN CELL; CONFERENCE PAPER; EXOCYTOSIS; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IMAGING; MEMBRANE VESICLE; NONHUMAN; SECRETORY VESICLE;

ANIMALIA; BOVINAE;

EID: 0037024687     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.2002.tb04472.x     Document Type: Conference Paper

References (5)

1. Avery, J. et al. 1999. Reconstitution of regulated exocytosis in cell-free systems: A critical appraisal. Annu. Rev. Physiol. 61: 777-807.
2. Avery, J. et al. 2000. A cell-free system for regulated exocytosis in PC 12 cells. J. Cell Biol. 148: 317-324.
3. Lang, T. et al. 2001. SNAREs are concentrated in cholesterol-dependent clusters that define docking and fusion sites for exocytosis. EMBO J. 20: 2202-2213.
4. Axelrod, D. et al. 1989. Total internal reflection fluorescence microscopy. Methods Cell Biol. 30: 245-270.
5. Duncan, R.R. et al. 1999. High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells. Biochem. J. 342: 497-501.