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Volumn 298, Issue 5591, 2002, Pages 213-216

Genetic loci affecting resistance to human malaria parasites in a West African mosquito vector population

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; GENES; GENETIC ENGINEERING; PATHOLOGY;

EID: 0037020123     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1073420     Document Type: Article
Times cited : (116)

References (35)
  • 7
  • 12
  • 13
    • 2142803437 scopus 로고    scopus 로고
    • Materials and methods are available as supporting material on Science Online.
    • Science Online
  • 14
    • 2142784971 scopus 로고    scopus 로고
    • note
    • Experimental protocols involving human subjects were approved by the ethical review boards of the New York University School of Medicine (protocol H-7270), the Faculté de Médecine, de Pharmacie et d'Odonto-Stomatologie of Mali (protocol 2001-0007), and the U.S. National Institutes of Health (protocol S-16077).
  • 15
    • 2142733178 scopus 로고    scopus 로고
    • note
    • Physical removal of unfed mosquitoes was verified at the time of dissection by determination that all mosquitoes displayed stages of ovarian development that require a blood meal. Thus, the absence of any oocysts resulted from failure of parasite development rather than lack of feeding.
  • 20
    • 2142790035 scopus 로고    scopus 로고
    • note
    • 1 family members. Mass mating was necessary because A. gambiae breed very inefficiently as isolated pairs. The program reads phenotype and genotype information and automatically performs phenotype distribution comparisons between mosquitoes that carry a specific genotype or allele against the pooled phenotype distribution of all other family members. Under the null hypothesis of no correlation between genotype and phenotype, phenotype distributions for a genotype or allele would be random picks from the overall phenotype distribution. The nominal P value reported here is the probability that the two distributions were randomly drawn from the same parent distribution. Nominal P values were computed using the KS test, and similar values were obtained using the Wilcoxon-Mann-Whitney ranking test.
  • 22
    • 2142675084 scopus 로고    scopus 로고
    • note
    • We performed a large number of tests per family and therefore had to correct for multiple testing, The standard Bonferroni correction (number of tests performed times nominal P value) is overly conservative because the tests are not independent: The markers are linked, multiple comparisons at the same locus are correlated, and not all DNA markers are equally informative. Instead of a Bonferroni correction, we used an empirical permutation test. We created replicates of the observed sample, in which genotype and phenotype distributions were preserved, but any linkage between genotype and phenotype was removed by randomly reassigning the observed phenotypic values. We then asked how often any replicates that conformed to the null hypothesis (the independence of genotype and phenotype) produced P values in excess of the P value observed for the real data. This test preserved both the observed marker informativeness and the overall phenotype distribution.
  • 24
    • 2142668954 scopus 로고    scopus 로고
    • note
    • Linkage of the resistance allele to two distinct marker alleles, 2 and 4, could be explained by recombination between resistance and marker alleles or by multiple entry of the resistance allele into the family. An attempt to distinguish by reconstructing flanking markers, although not conclusive, appeared more consistent with the recombination hypothesis.
  • 25
    • 0030890727 scopus 로고    scopus 로고
    • L. Zheng et al., Science 276, 425 (1997).
    • (1997) Science , vol.276 , pp. 425
    • Zheng, L.1
  • 26
    • 2142840453 scopus 로고    scopus 로고
    • S. F. Traoré, O. Niare, K. D. Vernick, data not shown
    • S. F. Traoré, O. Niare, K. D. Vernick, data not shown.
  • 29
    • 0035924332 scopus 로고    scopus 로고
    • C. L. Peichel et al., Nature 414, 901 (2001).
    • (2001) Nature , vol.414 , pp. 901
    • Peichel, C.L.1
  • 35
    • 2142837945 scopus 로고    scopus 로고
    • note
    • We acknowledge the support of O. Doumbo and the Equipe Parasitologique (FMPOS, Mali) and the organizational assistance of S. Karambe, R. Sakai, and R. Gwadz. Supported by a grant from the National Institute of Allergy and Infectious Disease/NIH to K.D.V., a DOE/Sloan Foundation Fellowship in Computational Molecular Biology to K.M., DFG Sonderforscungbereich 544 to F.C.K., a Ph.D. Fellowship from the University of Heidelberg to J.V., and a James S. McDonnell Centennial Fellowship to L.K.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.