Quantitative trait loci for refractoriness of Anopheles gambiae to Plasmodium cynomolgi B

Science

Volumn 276, Issue 5311, 1997, Pages 425-428

  Zheng, Liangbiao ^a   Cornel, Anton J ^b   Wang, Rui ^a   Erfle, Holger ^a   Voss, Hartmut ^a   Ansorge, Wilhelm ^a   Kafatos, Fotis C ^a   Collins, Frank H ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MELANIN;

ANOPHELES; ANTIMALARIAL ACTIVITY; ARTICLE; CONTROLLED STUDY; ENCAPSULATION; FEMALE; GENE LOCUS; MALARIA; MALARIA CONTROL; MALE; NONHUMAN; PLASMODIUM CYNOMOLGI; PRIORITY JOURNAL; QUANTITATIVE TRAIT;

ANIMALS; ANOPHELES; CHROMOSOME MAPPING; CROSSES, GENETIC; FEMALE; GENES, INSECT; GENOTYPE; INSECT VECTORS; LOD SCORE; MALE; MELANINS; MICROSATELLITE REPEATS; PHENOTYPE; PLASMODIUM CYNOMOLGI;

ANOPHELES GAMBIAE; PLASMODIUM CYNOMOLGI;

EID: 0030890727     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5311.425     Document Type: Article

References (27)

1. F. H. Collins et al., Science 234, 607 (1986).
2. M. Coluzzi et al., Trans. R. Soc. Trop. Med. Hyg. 73, 483 (1979).
3. K. D. Vernick and F. H. Collins, Am. J. Trop. Med. Hyg. 40, 593 (1989); A. E. Crews-Oyen et al., ibid. 49, 341 (1993).
4. K. D. Vernick and F. H. Collins, Am. J. Trop. Med. Hyg. 40, 593 (1989); A. E. Crews-Oyen et al., ibid. 49, 341 (1993).
5. a, Xag inversion of the X chromosome and the standard chromosome 3 karyotype.
6. F. H. Collins and A. J. Cornel, unpublished data.
7. 1 females were generated from a mass mating between L35 males and 4Ar/r females. Each was singly mated with one collared susceptible (either C/c or C/C), collarless susceptible (c/c), or collarless refractory male. These females were allowed to blood-feed on a P. cynomolgi B-infected rhesus monkey. After a single oviposition, the normal and encapsulated oocysts in the midgut of each female were counted 5 to 6 days later. The heterozygosity of some microsatellite markers in the susceptible strain allowed mapping of collarless in families E4 and E5 (7). Each BC family was individually reared, and the emerging females were allowed to blood-feed on another infected rhesus monkey. Infection and encapsulation phenotypes of the midgut were determined as above. The remaining carcasses were frozen for genomic DNA preparation and mi-crosatellite genotyping.
8. L. Zheng, F. H. Collins, V. Kumar, F. C. Kafatos, Science 261, 605 (1993); G. Dimopoulos et al., Genetics 143, 953 (1996); L. Zheng, M. Q. Benedict, A. Cornel, F. H. Collins, F. C. Kafatos, ibid., p. 941.
9. L. Zheng, F. H. Collins, V. Kumar, F. C. Kafatos, Science 261, 605 (1993); G. Dimopoulos et al., Genetics 143, 953 (1996); L. Zheng, M. Q. Benedict, A. Cornel, F. H. Collins, F. C. Kafatos, ibid., p. 941.
10. L. Zheng, F. H. Collins, V. Kumar, F. C. Kafatos, Science 261, 605 (1993); G. Dimopoulos et al., Genetics 143, 953 (1996); L. Zheng, M. Q. Benedict, A. Cornel, F. H. Collins, F. C. Kafatos, ibid., p. 941.
11. Fluorescent microsatellite genotyping was carried out as described [D. C. Mansfield et al., Genomics 24, 225 (1994)] with an ALF DNA sequencer (Pharmacia). The data were analyzed with Fragment Manager (Pharmacia). Genotyping of randomly selected mosquitoes was repeated to verify the quality of the data.
12. E. Lander et al., Genomics 1, 174 (1987); A. Paterson et al., Nature 336, 721 (1988).
13. E. Lander et al., Genomics 1, 174 (1987); A. Paterson et al., Nature 336, 721 (1988).
14. A genetic map was generated from the combined genotype data from families E1 to E5. QTL mapping was performed on individual and combined families E1 to E5 with the markers shown in Fig. 2 and for selected markers on E6 and E7. A LOD score of 3.0 was used as the minimum for declaring the existence of a QTL. After the highest value QTLs in chromosomes 2 and 3 (Pen1 and Pen2) were fixed, the residual variations were mapped, revealing only one additional significant QTL, Pen3. Maximum likelihood estimates were also calculated in families E1 to E7 for markers listed in Table 1 (9). A similar genetic map order was also obtained with the program Joinmap [P. Stam, Plant J. 5, 739 (1993)].
15. M. J. Gorman et al., Genetics, in press.
16. M. J. Gorman et al., Exp. Parasitol. 84, 380 (1996).
17. A. Warburg and L. H. Miller, Parasitol. Today 7, 179 (1991).
18. H. M. Al-Mashhadani, G. Davidson, C. F. Curtis, Trans. R. Soc. Trop. Med. Hyg. 74, 585 (1980); P. M. Graves and C. F. Curtis, Ann. Trop. Med. Parasitol 76, 633 (1982); M. Shahabuddin et al., Exp. Parasitol. 81, 386 (1995); K. D. Vernick et al., ibid. 80, 583 (1995).
19. H. M. Al-Mashhadani, G. Davidson, C. F. Curtis, Trans. R. Soc. Trop. Med. Hyg. 74, 585 (1980); P. M. Graves and C. F. Curtis, Ann. Trop. Med. Parasitol 76, 633 (1982); M. Shahabuddin et al., Exp. Parasitol. 81, 386 (1995); K. D. Vernick et al., ibid. 80, 583 (1995).
20. H. M. Al-Mashhadani, G. Davidson, C. F. Curtis, Trans. R. Soc. Trop. Med. Hyg. 74, 585 (1980); P. M. Graves and C. F. Curtis, Ann. Trop. Med. Parasitol 76, 633 (1982); M. Shahabuddin et al., Exp. Parasitol. 81, 386 (1995); K. D. Vernick et al., ibid. 80, 583 (1995).
21. H. M. Al-Mashhadani, G. Davidson, C. F. Curtis, Trans. R. Soc. Trop. Med. Hyg. 74, 585 (1980); P. M. Graves and C. F. Curtis, Ann. Trop. Med. Parasitol 76, 633 (1982); M. Shahabuddin et al., Exp. Parasitol. 81, 386 (1995); K. D. Vernick et al., ibid. 80, 583 (1995).
22. W. L. Kilama and G. B. Craig Jr., Ann. Trop. Med. Parasitol. 63, 419 (1969); D. W. Severson et al., Genetics 139, 1711 (1995).
23. W. L. Kilama and G. B. Craig Jr., Ann. Trop. Med. Parasitol. 63, 419 (1969); D. W. Severson et al., Genetics 139, 1711 (1995).
24. W. W. MacDonald and C. P. Ramachandran, Ann. Trop. Med. Parasitol. 59, 64 (1965); D. W. Severson et al., Insect Mol. Biol. 3, 67 (1994).
25. W. W. MacDonald and C. P. Ramachandran, Ann. Trop. Med. Parasitol. 59, 64 (1965); D. W. Severson et al., Insect Mol. Biol. 3, 67 (1994).
26. W.-J. Lee et al., unpublished data.
27. We thank S. Winkler, C. Schwager, R. Saffrich, U. Nentwich, and J. Stegemann for help in genotyping, and W. Collins, J. Sullivan, and C. Morris for help with rhesus monkeys. Supported by the John D. and Catherine T. MacArthur Foundation and the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR). Experiments with rhesus monkeys were performed under the guidelines of CDC animal use protocol 676-COL-MON-CH.