Penicillin amidase is activated for use in nonaqueous media by lyophilizing in the presence of potassium chloride

Enzyme and Microbial Technology

Volumn 31, Issue 3, 2002, Pages 193-197

  Lindsay, John P ^a   Clark, Douglas S ^b   Dordick, Jonathan S ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b University of California   (United States)

Author keywords

Nonaqueous media; Penicillin amidase; Salt activation; Transesterification

Indexed keywords

ACETONITRILE; CARBOHYDRATES; ESTERS; HYDRATION; POTASSIUM COMPOUNDS;

SALT ACTIVATION;

ENZYMES;

ACETONITRILE; BUFFER; HEXANE; ORGANIC SOLVENT; PENICILLIN AMIDASE; PENTANOL; PHENOXYACETIC ACID DERIVATIVE; PHENOXYACETIC ACID METHYL ESTER; PHOSPHATE; POTASSIUM CHLORIDE; PROPANOL; SODIUM CHLORIDE; TREHALOSE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVITY; FREEZE DRYING; HYDRATION; HYDROPHILICITY; TRANSESTERIFICATION;

EID: 0037008370     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00132-1     Document Type: Article

References (16)

1. Designing enzymes for use in organic solvents
2. Asymmetric transformations catalyzed by enzymes in organic solvents
3. Effects of ester synthesis in toluene by immobilized chymotrypsin by addition of polymers to reaction medium
4. Effect of the enzyme form on the activity, stability and enantioselectivity of lipoprotein lipase in toluene
5. Dramatic enhancement of enzymic activity in organic solvents by lyoprotectants
6. Large activation of serine proteases by pretreatment with crown ethers
7. Effects of crown ethers and small amounts of cosolvent on the activity and enantioselectivity of alpha-chymotrypsin in organic solvents
8. Salts dramatically enhance activity of enzymes suspended in organic solvents
9. On the salt-induced activation of lyophilized enzymes in organic solvents: Effect of salt kosmotropicity on enzyme Activity
10. Synthesis of water-soluble paclitaxel derivatives by enzymic acylation
11. The role of penicillin amidases in nature and in industry [published erratum appears in Trends Biochem Sci 1991;16(5):194]
12. Substrate specificity of penicillin amidase from E. Coli
13. Organic solvents strip water off enzymes
14. The effect of water on enzyme action in organic media
15. Papain in organic solvents: Determination of conditions suitable for biocatalysis and the effect on substrate specificity and inhibition
16. Separation of freezing- and drying-induced denaturation of lyophilized proteins using stress-specific stabilization. II. Structural studies using infrared spectroscopy