Tauroursodeoxycholic acid mobilizes α-PKC after uptake in human HepG2 hepatoma cells

European Journal of Clinical Investigation

Volumn 32, Issue 6, 2002, Pages 437-442

  Glasova, Helena ^a   Berghaus, Thomas M ^a   Kullak Ublick, Gerd A ^b   Paumgartner, Gustav ^a   Beuers, Ulrich ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

Bile acids; Cholestasis; Exocytosis; HepG2 cells; Na+ taurocholate cotransporting polypeptide; Tauroursodeoxycholic acid

Indexed keywords

CARRIER PROTEIN; COMPLEMENTARY DNA; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C ALPHA; PROTEIN NTCP; TAUROCHOLIC ACID; TAUROURSODEOXYCHOLIC ACID; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE; CELL STRAIN HEPG2; CELL TRANSPORT; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DRUG MECHANISM; DRUG UPTAKE; ENZYME ASSAY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; ISOTOPE LABELING; LIVER CELL; MEMBRANE BINDING; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; WESTERN BLOTTING;

BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELL FRACTIONATION; HEPATOCYTES; HUMANS; ISOENZYMES; MEMBRANE TRANSPORT PROTEINS; ORGANIC ANION TRANSPORTERS, SODIUM-DEPENDENT; PROTEIN KINASE C; PROTEIN KINASE C-ALPHA; SYMPORTERS; TAUROCHENODEOXYCHOLIC ACID; TETRADECANOYLPHORBOL ACETATE; TRANSFECTION; TRITIUM; TUMOR CELLS, CULTURED;

EID: 0037000742     PISSN: 00142972     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2362.2002.01002.x     Document Type: Article

References (27)

1. Poupon RE, Lindor KD, Cauch-Dudek K, Dickson ER, Poupon R, Heathcote EJ. Combined Analysis of Randomized Controlled Trials of Ursodeoxycholic Acid in Primary Biliary Cirrhosis. Gastroenterology 1997;113:884-90.
2. Beuers U, Paumgartner G, Boyer JL. Ursodeoxycholic acid in cholestasis: Potential mechanisms of action and therapeutic applications. Hepatology 1998;28:1449-53.
3. Trauner M, Graziadei IW. Mechanisms of action and therapeutic applications of ursodeoxycholic acid in chronic liver disease. Aliment Pharmacol Ther 1999;13:979-96.
4. Bouscarel B, Kroll SD, Fromm H. Signal transduction and hepatocellular bile acid transport. Cross talk between bile acids and second messengers. Gastroenterology 1999;117:433-52.
5. ++ mechanisms defective in cholestasis. J Clin Invest 1993;92:2984-93.
6. Beuers U, Throckmorton DC, Anderson MS, Isales CM, Thasler W, Kullak-Ublick GA et al. Tauroursodeoxycholic acid activates protein kinase C in isolated rat hepatocytes. Gastroenterology 1996;110:1553-63.
7. ++ signals in isolated rat hepatocytes. Gastroenterology 1993;104:604-12.
8. ++ and activates phosphorylase a in isolated hepatocytes. Am J Physiol 1993;264:G243-51.
9. Beuers U, Bilzer M, Chittattu A, Kullak-Ublick GA, Keppler D, Paumgartner G et al. Tauroursodeoxycholic acid inserts the apical conjugate export pump Mrp2 into canalicular membranes and stimulates organic anion secretion by protein kinase C-dependent mechanisms in cholestatic rat liver. Hepatology 2001;33:1206-16.
10. Larkin JM, Palade GE. Transcytotic vesicular carriers for polymeric IgA receptors accumulate in rat hepatocytes after total biliary obstruction. J Cell Sci 1991;98:205-16.
11. Crawford JM. Role of vesicle-mediated transport pathways in hepatocellular bile secretion. Semin Liver Dis 1996;16:169-89.
12. ++-stimulated secretion by modulating membrane-attached exocytic machinery. J Biol Chem 1999;274:26469-76.
13. Bouscarel B, Gettys TW, Fromm H, Dubner H. Ursodeoxycholic acid inhibits glucagon-induced cAMP formation in hamster hepatocytes: a role for PKC. Am J Physiol 1995;268:G300-10.
14. Stravitz RT, Rao YP, Vlahcevic ZR, Gurley EC, Jarvis WD, Hylemon PB. Hepatocellular protein kinase C activation by bile acids: implications for regulation of cholesterol 7 (-hydroxylase). Am J Physiol 1996;271:G293-G303.
15. Müller M, Jansen PL. The secretory function of the liver: new aspects of hepatobiliary transport. J Hepatol 1998;28:344-54.
16. Kullak-Ublick GA, Beuers U, Paumgartner G. Hepatobiliary transport. J Hepatol 2000;32 (Suppl. 1):3-18.
17. Kullak-Ublick GA, Beuers U, Paumgartner G. Molecular and functional characterization of bile acid transport in human hepatoblastoma HepG2 cells. Hepatology 1996;23:1053-60.
18. +-taurocholate cotransporting green fluorescent protein in human hepatoblastoma HepG2 cells. Cytotechnology 2000;34:1-9.
19. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
20. Beuers U, Probst I, Soroka C, Boyer JL, Kullak-Ublick GA, Paumgartner G. Modulation of protein kinase C by taurolithocholic acid in isolated rat hepatocytes. Hepatology 1999;29:477-82.
21. Crosignani A, Podda M, Battezzati PM, Bertolini R, Zuin M, Watson D et al. Changes in bile acid composition in patients with primary biliary cirrhosis induced by ursodeoxycholic acid administration. Hepatology 1991;14:1000-7.
22. Beuers U, Spengler U, Zwiebel FM, Pauletzki J, Fischer S, Paumgartner G. Effect of ursodeoxycholic acid on the kinetics of the major hydrophobic bile acids in health and in chronic cholestatic liver disease. Hepatology 1992;15:603-8.
23. ++ increase in human neutrophils stimulated by formyl-methionyl-leucyl-phenylalanine. Biochem Biophys Res Commun 1990;171:1115-21.
24. ++ activity of isolated rat hepatocytes. Hepatology 1988;8:887-91.
25. ++ in rat hepatocytes. Eur J Biochem 1990;190:619-23.
26. 2+ in rat hepatocytes, human platelets and neuroblastoma NG108-15 cell line. Biochem J 1991;273:153-60.
27. Noe J, Hagenbuch B, Meier PJ, St-Pierre MV. Characterization of the mouse bile salt export pump overexpressed in the baculovirus system. Hepatology 2001;33:1223-31.