Use of fluorescence in situ hybridization for gross mapping of transgenes and screening for homozygous plants in transgenic barley (Hordeum vulgare L.)

Theoretical and Applied Genetics

Volumn 106, Issue 1, 2002, Pages 92-100

  Choi, H W ^a   Lemaux, P G ^a   Cho, M J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Barley; Fluorescence in situ hybridization; Homozygosity; Transgene expression; Transgene localization

Indexed keywords

DNA; FLUORESCENCE; PLANTS (BOTANY);

TRANSGENIC PLANTS;

GENES;

EMBRYOPHYTA; HORDEUM; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE;

ACYLTRANSFERASE; PHOSPHINOTHRICIN N ACETYLTRANSFERASE; PHOSPHINOTHRICIN N-ACETYLTRANSFERASE;

ARTICLE; BARLEY; CHROMOSOME MAP; FLUORESCENCE IN SITU HYBRIDIZATION; GENE DOSAGE; GENE EXPRESSION; GENETICS; HOMOZYGOTE; PHENOTYPE; POLYPLOIDY; TRANSGENE; TRANSGENIC PLANT;

ACETYLTRANSFERASES; CHROMOSOME MAPPING; GENE DOSAGE; GENE EXPRESSION; HOMOZYGOTE; HORDEUM; IN SITU HYBRIDIZATION, FLUORESCENCE; PHENOTYPE; PLANTS, GENETICALLY MODIFIED; POLYPLOIDY; TRANSGENES;

EID: 0036987749     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00122-002-0997-y     Document Type: Article

References (43)

1. Abranches R, Santos AP, Wegel E, Williams S, Castilho A, Christou P, Shaw P, Stoger E (2000) Widely separated multiple transgene integration sites in wheat chromosomes are brought together at interphase. Plant J 24:713-723
2. Ambros PF, Matzke MA, Matzke AJM (1986a) Detection of a 17-kb unique sequence (T-DNA) in plant chromosomes by in situ hybridization. EMBO J 5:2073-2077
3. Ambros PF, Matzke MA, Matzke AJM (1986b) Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization. Chromosoma 94:11-18
4. Carlson AR, Letarte J, Chen J, Kasha KJ (2001) Visual screening of microspore-derived transgenic barley (Hordeum vulgate L.) with green-fluorescent protein. Plant Cell Rep 20:331-337
5. Cho M-J, Jiang W, Lemaux PG (1998) Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism. Plant Sci 38:229-244
6. Cho M-J, Choi HW, Buchanan BB, Lemaux PG (1999) Inheritance of tissue-specific expression of hordein promoter-uidA fusions in transgenic barley plants. Theor Appl Genet 98:1253-1262
7. Cho M-J, Ha CD, Lemaux PG (2000) Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues. Plant Cell Rep 19:1084-1089
8. Cho M-J, Choi HW, Jiang W, Ha CD, Lemaux PG (2002) Endosperm-specific expression of green fluorescent protein is stably inherited in transgenic barley (Hordeum vulgate L.) plants. Physiol Plant 115:144-154
9. Choi HW, Lemaux PG, Cho M-J (2000) Increased chromosomal variation in transgenic versus nontransgenic barley (Hordeum wdgare L.) plants. Crop Sci 40:524-533
10. Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5:213-218
11. Dellaporta S (1994) Plant DNA miniprep and microprep. In: Freeling M, Walbot V (eds) Maize handbook. Springer, New York, pp 522-525
12. Finnegan JJ, McElroy D (1994) Transgene inactivation: plants fight back! Bio/Technology 12:883-888
13. Flavell RB (1994) Inactivation of gene expression in plants as a consequence of novel sequence duplications. Proc Natl Acad Sci USA 91:3490-3496
14. Fransz PF, Stam M, Montijn BM, Hoopen RT, Wiegant J, Kooter JM, Oud O, Nanningga N (1996) Detection of single-copy genes and chromosome rearrangements in Petunia hybrida by fluorescence in situ hybridization. Plant J 9:676-774
15. Fukui K, Kakeda K (1990) Quantitative karyotyping of barley chromosomes by image analysis methods. Genome 33:450-458
16. Gall JG, Pardue ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63:378-383
17. Gerlach WT, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 8:1869-1885
18. Hoopen RT, Robbins TP, Fransz PF, Montijn BM, Oud O, Gerats AGM, Nanningga N (1996) Localization of T-DNA insertions in Petunia by fluorescence in situ hybridization: physical evidence for suppression of recombination. Plant Cell 8:823-830
19. Islam-Faradi MN, Mujeeb-Kazi A (1995) Visualization of Secale cereale DNA in wheat germ plasm by fluorescent in situ hybridization. Theor Appl Genet 90:595-600
20. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901-3907
21. Jiang J, Gill BS (1994) Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37:717-725
22. John HA, Birnstiel ML, Jones KW (1969) RNA-DNA hybrids at the cytological level. Nature 223:582-587
23. Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci USA 95:7203-7208
24. Leggett JM, Perret S J, Harper J, Morris P (2000) Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization. Heredity 84:46-53
25. Leitch IJ, Heslop-Harrison JS (1992) Physical mapping of the 18S-5.8S-26S rRNA genes in barley by in situ hybridization. Genome 35:1013-1018
26. Lemaux PG, Cho M-J, Louwerse J, Williams R, Wan Y (1996) Bombardment-mediated transformation methods for barley. Bio-Rad Bull 2007:1-6
27. Linde-Laursen I, Heslop-Harrison JS, Shepherd KW, Taketa S (1997) The barley genome and its relationship with the wheat genomes. A survey with an internationally agreed recommendation for barley chromosome nomenclature. Hereditas 126:1-16
28. Moscone EA, Matzke MA, Matzke AJM (1996) The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploid tobacco. Chromosoma 105:231-236
29. Mouras A, Negrutiu (1989) Localization of the T-DNA on marker chromosomes in transfomed tobacco cells by in situ hybridization. Theor Appl Genet 78:715-720
30. Mouras A, Saul MW, Essad S, Potrykus I (1987) Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer. Mol Gen Genet 207:204-209
31. Noda K, Kasha KJ (1978) A proposed barley karyotype revision based on C-band chromosome identification. Crop Sci 18:925-930
32. Ohmido N, Akiyama Y, Fukui K (1998) Physical mapping of unique nucleotide sequences on identified rice chromosomes. Plant Mol Biol 38:1043-1052
33. Pedersen C, Zimny J, Becker D, Jähne-Gärtner, Lörz H (1997) Localization of introduced genes on the chromosomes of transgenic barley, wheat and triticale by fluorescence in situ hybridization. Theor Appl Genet 94:749-757
34. Phillips RL, Kaeppler SM, Olhoft P (1994) Genetic instability of plant tissue cultures: breakdown of normal controls. Proc Natl Acad Sci USA 91:5222-5226
35. Rayburn AL, Gill BS (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered 76:78-81
36. Salvo-Garrido H, Travell S, Schwarzacher T, Harwood WA, Snape JW (2001) An efficient method for physical mapping of transgenes in barley using in situ hybridization. Genome 44:104-110
37. Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64:315-324
38. Sørensen MB, Müller M, Skerrit J, Simpson D (1996) Hordein promoter methylation and transcriptional activity in wild-type and mutant barley endosperm. Mol Gen Genet 250:750-760
39. Stam M, Mol JNM, Kooter JM (1997) The silence of genes in transgenic plants. Ann Bot 79:3-12
40. Svitashev S, Ananiev E, Pawlowski WP, Somers DA (2000) Association of transgene integration sites with chromosome rearrangements in hexaploid oat. Theor Appl Genet 100:872-880
41. Vasil IK (1994) Molecular improvement of cereals. Plant Mol Biol 25:925-937
42. Wallroth M, Gerats AGM, Rogers SG, Fraley RT, Horsch RB (1986) Chromosomal localization of foreign genes in Petunia hybrida. Mol Gen Genet 202:6-15
43. Wang J, Lewis ME, Whallon JH, Sink KC (1995) Chromosomal mapping of T-DNA inserts in transgenic Petunia by in situ hybridization. Transgenic Res 4:241-246