Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation

Journal of Bacteriology

Volumn 184, Issue 24, 2002, Pages 6859-6865

  Kosugi, Akihiko ^a   Murashima, Koichiro ^b   Doi, Roy H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b MEIJI SEIKA KAISHA LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA ARABINOFURANOSIDASE; ALPHA ARABINOPYRANOSIDASE; ARABINOXYLAN; ARFA PROTEIN; BACTERIAL ENZYME; BACTERIAL PROTEIN; BETA GALACTOSIDASE; BGAA PROTEIN; GLYCOSIDASE; HEMICELLULOSE; POLYMER; PYRANOSIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CLOSTRIDIUM; CLOSTRIDIUM CELLULOVORAN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME DEGRADATION; ENZYME SUBSTRATE; GENE ISOLATION; GENE LIBRARY; GENE LOCATION; GENE STRUCTURE; GENETIC ANALYSIS; MOLECULAR CLONING; MOLECULAR WEIGHT; MULTIGENE FAMILY; NONHUMAN; PLANT CELL; PRIORITY JOURNAL; PROTEIN BINDING;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELL WALL; CLONING, MOLECULAR; CLOSTRIDIUM; GLYCOSIDE HYDROLASES; PLANTS; POLYSACCHARIDES; POLYSACCHARIDES, BACTERIAL; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM CELLULOVORANS; POSIBACTERIA;

EID: 0036952134     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.24.6859-6865.2002     Document Type: Article

References (32)

1. Aspinall, G. O. 1980. Chemistry of cell-wall polysaccharides, p. 473-500. In J. Press (ed.), The biochemistry of plants, vol. 3. Academic Press, Inc., New York, N.Y.
2. Beylot, M.-H., V. A. McKie, A. G. Voragen, C. H. Doeswijk-Voragen, and H. J. Gilbert. 2001. The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity. Biochem. J. 358:607-614.
3. Bezalel, L., Y. Shoham, and E. Rosenberg. 1993. Characterization and delignification activity of a thermostable α-L-arabinofuranosidase from Bacillus stearothermophilus. Appl. Microbiol. Biotechnol. 40:57-62.
4. Coombs. J. M., and J. E. Brenchley. 1999. Biochemical and phylogenetic analysis of a cold-active β-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA. Appl. Environ. Microbiol. 65:5443-5450.
5. Debeche, T., N. Cummings, I. Connerton, P. Debeire, and M. J. O'Donohue. 2000. Genetic and biochemical characterization of a highly thermostable α-L-arabinofuranosidase from Thermobacillus xylanilyticus. Appl. Environ. Microbiol. 66:1734-1736.
6. de Vries, R. P., H. C. M. Kester, C. H. Poulsen, J. A. E. Benen, and J. Visser. 2000. Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides. Carbohydr. Res. 327:401-410.
7. Doi, R. H., and Y. Tamaru. 2001. The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity. Chem. Rec. 1:24-32.
8. Doner, W. L., D. B. Johnston, and V. Singh. 2001. Analysis and properties of arabinoxylans from discrete corn wet-milling fiber fractions. J. Agric. Food Chem. 49:1266-1269.
9. Gilead, S., and Y. Shohan. 1995. Purification and characterization of α-L-arabinofuranosidase from Bacillus stearothermophilus T-6. Appl. Environ. Microbiol. 61:170-174.
10. Goldstein, M. A., and R. H. Doi. 1995. Prokaryotic promoters in biotechnology. Biotechnol. Annu. Rev. 1:105-128.
11. Gutshall, K. R., D. E Trimbur, J. J. Kasmir, and J. E. Brenchley. 1995. Analysis of a novel gene and β-galactosidase isozyme from a psychrotrophic Arthrobacter isolate. J. Bacteriol. 177:1981-1988.
12. Holmes, M. L., and M. L. Dyall-Smith. 2000. Sequence and expression of a halobacterial β-galactosidase gene. Mol. Microbiol. 36:114-122.
13. Hung, M.-N., Z. Xia, N.-T. Hu, and B. H. Lee. 2001. Molecular and biochemical analysis of two β-galactosidases from Bifidobacterium infantis HL96. Appl. Environ. Microbiol. 67:4256-4263.
14. Kim, K. S., T. G. Lilburn, M. J. Renner, and J. A. Breznak. 1998. arfI and arfII, two genes encoding α-L-arabinofuranosidases in Cytophaga xylanolytica. Appl. Environ. Microbiol. 64:1919-1923.
15. Kosugi, A., K. Murashima, and R. H. Doi. 2001. Characterization of xylanolytic enzymes in Clostridium cellulovorans: expression of xylanase activity dependent on growth substrates. J. Bacteriol. 183:7037-7043.
16. Lachke, A. H. 1988. 1,4-β-D-Xylan xylohydrolase of Sclerotium rolfsii. Methods Enzymol. 160:679-684.
17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 277:680-685.
18. Matsuo, N., S. Kaneko, A. Kuno, H. Kobayashi, and I. Kusakabe. 2000. Purification, characterization and gene cloning of two α-L-arabinofuranosidases from Streptomyces chartreusis GS901. Biochem. J. 346:9-15.
19. Moller, P. L., F. Jorggensen, O. C. Hansen, S. M. Madsen, and P. Stougaard. 2001. Intra- and extracellular β-galactosidases from Bifidobacterium bifidum and B. infantis: molecular cloning, heterologous expression, and comparative characterization. Appl. Environ. Microbiol. 67:2276-2283.
20. Murashima, K., A. Kosugi, and R. H. Doi. 2002. Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls. Appl. Environ. Microbiol. 68:1610-1615.
21. Saitou, N., and M. Nei. 1987. The neighbor-joining method, a new method for constructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
22. Schwarz, W. H., K. Bronnenmeier, B. Krause, F. Lottspeich, and W. L. Staudenbauer. 1995. Debranching of arabinoxylan: properties of the thermoactive recombinant α-L-arabinofuranosidase from Clostridium stercorarium (ArfB). Appl. Microbiol. Biotechnol. 43:856-860.
23. Sheridan, P. P., and J. E. Brenchley. 2000. Characterization of a salt-tolerant family 42 β-galactosidase from a psychrophilic Antarctic Planococcus isolate. Appl. Environ. Microbiol. 66:2438-2444.
24. Shoham, Y., Z. Schwartz, A. Khasin, O. Gat, Z. Zosim, and E. Rosenberg. 1993. Delignification of wood pulp by thermostable xylanase from Bacillus stearothermophilus T-6. Biodegradation 3:207-218.
25. Sleat, R., R. A. Mah, and R. Robinson. 1984. Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium cellulovorans sp. nov. Appl. Environ. Microbiol. 48:88-93.
26. Tamaru, Y., and R. H. Doi. 1999. Three surface layer homology domains at the N terminus of the Clostridium cellulovorans major cellulosomal subunit EngE. J. Bacteriol. 181:3270-3276.
27. Tamaru, Y., S. Ui, K. Murashima, A. Kosugi, H. Chan, R. H. Doi, and B. Liu. 2002. Formation of protoplasts from cultured tobacco cells and Arabidopsis thaliana by the action of cellulosomes and pectate lyase from Clostridium cellulovorans. Appl. Environ. Microbiol. 68:2614-2618.
28. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
29. Thomson, J. A. 1993. Molecular biology of xylan degradation. FEMS Microbiol. Rev. 104:65-82.
30. Volkin, D. B., and A. M. Klibanov. 1989. Minimizing protein inactivation, p. 1-24. In T. E. Creighton (ed.), Protein function, a practical approach. IRL Press, Oxford, England.
31. Wood, W. A., and K. M. Bhat. 1988. Methods for measuring cellulose activities. Methods Enzymol. 160:87-112.
32. Zverlov, V. V., W. Liebl, M. Bachleitner, and W. H. Schwarz. 1998. Nucleotide sequence of arfB of Clostridium stercorarium, and prediction of catalytic residues of α-L-arabinofuranosidases based on local similarity with several families of glycosyl hydrolases. FEMS Microbiol. Lett. 164:337-343.