Formation of protoplasts from cultured tobacco cells and Arabidopsis thaliana by the action of cellulosomes and pectate lyase from Clostridium cellulovorans

Applied and Environmental Microbiology

Volumn 68, Issue 5, 2002, Pages 2614-2618

  Tamaru, Yutaka ^a   Ui, Sadaharu ^a   Murashima, Koichiro ^a   Kosugi, Akihiko ^a   Chan, Helen ^a   Doi, Roy H ^a   Liu, Bo ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; PLANT CELL CULTURE; TOBACCO;

CRUDE CULTURE SUPERNATANTS;

MICROORGANISMS;

ALGAROBA; BACTERIAL ENZYME; CELLOBIOSE; CELLULOSOME; GLUCOSE; PECTATE LYASE; PECTIN; UNCLASSIFIED DRUG; XYLAN;

BACTERIUM;

ARABIDOPSIS; ARTICLE; CELL TRANSFORMATION; CLOSTRIDIUM; CLOSTRIDIUM CELLULOVORANS; COCULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; NONHUMAN; PLANT CELL CULTURE; PROTOPLAST; SUPERNATANT; TOBACCO;

ARABIDOPSIS; CARBON; CELLULASE; CLOSTRIDIUM; POLYSACCHARIDE-LYASES; PROTOPLASTS; TOBACCO;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM CELLULOVORANS; NICOTIANA TABACUM; PHASEOLUS (ANGIOSPERM); POSIBACTERIA;

EID: 0036249969     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.5.2614-2618.2002     Document Type: Article

References (22)

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2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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5. Analysis of functional domains of endoglucanases from Clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction
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7. Partial purification of a cellulase used for protoplast isolation from barley (Hordeum vulgare)
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9. Synthesis, assembly and function of plant cell wall macromolecules
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14. Tobacco BY-2 cell line as the "HeLa" cell in the cell biology of higher plants
15. Characterization of engF, a gene for a non-cellulosomal Clostridium cellulovorans endoglucanase
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