Functional tolerance of Streptomyces subtilisin inhibitor toward conformational and stability changes caused by single-point mutations in the hydrophobic core

Journal of Biochemistry

Volumn 132, Issue 6, 2002, Pages 991-995

  Oda, Masayuki ^a   Tamura, Atsuo ^b   Kanaori, Kenji ^c   Kojima, Shuichi ^d   Miura, Kin ichiro ^d   Momma, Keiko ^e   Tonomura, Ben'ichiro ^e   Akasaka, Kazuyuki ^f  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

^b KOBE UNIVERSITY   (Japan)

^c KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^d GAKUSHUIN UNIVERSITY   (Japan)

^e KYOTO UNIVERSITY   (Japan)

^f KINKI UNIVERSITY   (Japan)

Author keywords

Conformational change; Inhibitory function; Protein engineering; Streptomyces subtilisin inhibitor; Thermal stability

Indexed keywords

AMINO ACID; SERINE PROTEINASE INHIBITOR; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME INHIBITION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; HYDROPHOBICITY; NONHUMAN; POINT MUTATION; PROTEIN FUNCTION; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; STREPTOMYCES; STRESS; STRUCTURE ANALYSIS; THERMODYNAMICS;

STREPTOMYCES;

EID: 0036952130     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003314     Document Type: Article

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