Transgene behaviour across two generations in a large random population of transgenic rice plants produced by particle bombardment

Theoretical and Applied Genetics

Volumn 105, Issue 6-7, 2002, Pages 878-889

  Vain, Philippe ^a   James, V A ^a   Worland, B ^a   Snape, J W ^a  

^a JOHN INNES CENTRE   (United Kingdom)

Author keywords

Attachment Regions; Fertility; Matrix; Oryza sativa; Particle bombardment; Transgene expression

Indexed keywords

DNA; GENES;

PARTICLE BOMBARDMENT;

PLANTS (BOTANY);

EMBRYOPHYTA; ORYZA SATIVA;

EID: 0036944933     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00122-002-1039-5     Document Type: Article

References (36)

1. Allen GC, Spiker S, Thompson WF (2000) Use of matrix attachment regions (MARs) to minimize transgene silencing. Plant Mol Biol 43:361-376
2. Bhattacharyya M, Stemer BA, Dixon RA (1994) Reduced variation in transgene expression from a binary vector with selectable markers at the right and left T-DNA borders. Plant J 6:957-968
3. Breitler JC, Cordero MJ, Royer M, Meynard D, Segundo BS, Guideroni E (2002) The -689/+197 region of the maize protease inhibitor gene directs high level, wound-inducible expression of the cry1B gene which protects transgenic rice plants from stemborer attack. Theor Appl Genet (in press)
4. Breyne P, Gheysen G, Jacobs A, Van Montagu M, Depicker A (1992) Effect of T-DNA configuration on transgene expression. Mol Gen Genet 235:389-396
5. Christou P (1996) Transformation technology. Trends Plant Sci 1:423-431
6. Finnegan J, McElroy D (1994) Transgene inactivation: Plants fight back. Bio/Technology 12:883-888
7. Fu X, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sudhakar D, Twyman RM, Christou P, Kohli A (2000) Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res 9:11-19
8. Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR Jr, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2:603-618
9. Hansen G, Chilton M-D (1996) "Agrolistic" transformation of plant cells: Integration of T-strands generated in planta. Proc Natl Acad Sci USA 93:14,978-14,983
10. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries T-DNA. Plant J 6:271-282
11. Jefferson RA, Kavanagh TA, Bevan MW (1987) β-glucuronidase as a sensitive and versatile fusion marker in higher plants. EMBO J 6:3901-3907
12. Iyer LM, Kumpatla SP, Chandrasekharan MB, Hall TC (2000) Transgene silencing in monocots. Plant Mol Biol 43:323-346
13. James VA, Avart C, Worland B, Snape JW, Vain P (2002) The relationship between homozygous and hemizygous transgene expression levels over generations in populations of transgenic rice plants. Theor Appl Genet 104:553-561
14. Jones JDG, Dunsmuir D, Bedbrook J (1985) High level expression of introduced chimearic genes in regenerated transformed plants. EMBO J 10:2411-2418
15. Kohli A, Griffiths S, Palacios N, Twyman RM, Vain P, Laurie DA, Christou P (1999) Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology mediated recombination. Plant J 17:591-601
16. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10:165-174
17. Kumpatla SP, Hall TC (1998) Recurrent onset of epigenetic silencing in rice harboring a multi-copy transgene. Plant J 14:129-135
18. Linn F, Heidmann I, Saedler H, Meyer P (1990) Epigenetic changes in the expression of the maize Al gene in Petunia hybrida: Role of numbers of integrated gene copies and methylation state. Mol Gen Genet 222:329-336
19. Lyznik LA, Ryan RD, Ritchie SW, Hodges TK (1989) Stable co-transformation of maize protoplasts with gusA and neo genes. Plant Mol Biol 13:151-161
20. Matzke AJM, Matzke MA (1998) Position effect and epigenetic silencing of plant transgenes. Curr Opin Plant Biol 1:142-148
21. Meyer P (1995) Understanding and controlling transgene expression. Trends Biotechnol 13:332-337
22. Mirkovitch J, Mirault ME, Laemmli UK (1984) Organization of the higher-order chromatin loop: DNA attachment sites on nuclear scaffold. Cell 39:223-232
23. Mlynárová L, Loonen A, Heldens J, Jansen RC, Keizer P, Stiekema WJ, Nap JP (1994) Reduced position effect in mature transgenic plants conferred by the chicken lysozyme matrix-associated region. Plant Cell 6:417-426
24. Mlynárová L, Jansen RC, Conner AJ, Stiekema WJ, Nap JP (1995) The MAR-mediated reduction in position effect can be uncoupled from copy number-dependent expression in transgenic plants. Plant Cell 7:599-609
25. Pazkowski J, Baur M, Bogucki A, Potrykus I (1988) Gene targeting in plants. EMBO J 7:4021-4026
26. Pawlowski WP, Somers DA (1996) Transgene inheritance in plants genetically engineered by microprojectile bombardment. Mol Biotechnol 6:17-30
27. Peach C, Velten J (1991) Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol Biol 17:49-60
28. Register JC III, Peterson DJ, Bell PJ, Bullock WP, Evans IJ, Frame B, Greenland AJ, Higgs NS, Jepson I, Jiao S, Lewnau CJ, Sillick JM, Wilson HM (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25:951-961
29. Srivastava V, Anderson OD, Ow DW (1999) Single-copy transgenic wheat generated through the resolution of complex integration patterns. Proc Natl Acad Sci USA 96:11,117-11,121
30. Svitashev SK, Somers DA (2001) Genomic interspersions determine the size and complexity of transgene loci in transgenic plants produced by microprojectile bombardment. Genome 44:691-697
31. Takano M, Egawa H, Ikeda JE, Wakasa K (1997) The structures of integration sites in transgenic rice. Plant J 11:353-361
32. Ülker B, Allen GC, Thompson WF, Spiker S, Weissinger AK (1999) A tobacco MAR increases transgene expression and protects against gene silencing in the progeny of transgenic tobacco plants. Plant J 18:253-263
33. Vain P, Worland B, Clarke MC, Richard G, Beavis M, Liu H, Kohli A, Leech M, Snape J, Christou P, Atkinson H (1998) Expression of an engineered proteinase inhibitor (Oryzacystatin-IΔd86) for nematode resistance in transgenic rice plants. Theor Appl Genet 96:266-271
34. Vain P, Worland B, Kohli A, Snape JW, Christou P, Allen GC, Thompson WF (1999) Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny. Plant J 18:233-242
35. Wan Y, Lemaux PG (1994) Generation of large numbers of independently transformed fertile barley plants. Plant Physiol 104:37-48
36. Walters DA, Vetsch CS, Potts DE, Lundquist RC (1992) Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Plant Mol Biol 18:189-200