Nitrogen stress response of Prochlorococcus strain PCC 9511 (Oxyphotobacteria) involves contrasting regulation of ntcA and amt1

Journal of Phycology

Volumn 38, Issue 6, 2002, Pages 1113-1124

  Lindell, Debbie ^a,g   Erdner, Deana ^b   Marie, Dominique ^c   Prášil, Ondřej ^d,e   Koblížek, Michal ^d,e   Le Gall, Florence ^c   Rippka, Rosmarie ^f   Partensky, Frédéric ^c   Scanlan, David J ^b   Post, Anton F ^a  

^a The Interuniversity Institute for Marine Science Eilat   (Israel)

^b UNIVERSITY OF WARWICK   (United Kingdom)

^c STATION BIOLOGIQUE   (France)

^d INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^e UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

^f CNRS   (France)

^g MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Ammonium transporter; Nitrogen regulation; Nitrogen stress; Photosynthetic activity; Picophytoplankton; Prochlorococcus

Indexed keywords

NITROGEN; PHYSIOLOGICAL RESPONSE; STRESS;

CYANOBACTERIA; PROCHLOROCOCCUS;

EID: 0036919824     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1529-8817.2002.01205.x     Document Type: Article

References (35)

1. Alfonso, M., Perewoska, I. & Kirilovsky, D. 2001. Redox control of ntcA gene expression in Synechocystis sp. PCC 6803. Nitrogen availability and electron transport regulate the levels of the NtcA protein. Plant Physiol. 125:969-81.
2. Chisholm, S. W., Olson, R. J., Zettler, E. R., Goericke, R., Waterbury, J. B. & Welschmeyer, N. A. 1988. A novel free-living prochlorophyte abundant in the oceanic enphotic zone. Nature 334:340-3.
3. El Alaoui, S., Diez, J., Humanes, L., Toribio, F., Partensky, F. & Garcia-Fernandez, J. M. 2001. In vivo regulation of glutamine synthetase activity in the marine chlorophyll b-containing cyanobacterium Prochlorococcus sp. strain PCC 9511 (oxyphotobacteria) Appl. Environ. Microbiol. 67:2202-7.
4. Garcia-Fernandez, J. M., Hess, W. R., Houmard, J. & Partensky, F. 1998. Expression of the psbA gene in the marine oxyphotobacteria Prochlorococcus spp. Arch. Biochem. Biophys. 359:17-23.
5. Herrero, A., Muro-Pastor, A. M. & Flores, E. 2001. Nitrogen control in cyanobacteria. J. Bacteriol. 183:411-25.
6. Holmes, R. M., Aminot, A., Kerouel, R., Hooker B. A. & Peterson, B. J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish Aquat. Sci. 56:1801-8.
7. Jakoby, M., Nolden, L., Meier-Wagner, J., Krämer, R. & Burkovski, A. 2000. AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum. Mol. Microbiol. 37:964-77.
8. -1 on marine Synechococcus WH 7803. I. Growth, pigmentation and cell composition. Deep-Sea Res. 34:479-95.
9. 11 (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 181:2697-702.
10. Lindell, D., Padan, E. & Post, A. F. 1998. Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. strain WH 7803. J. Bacteriol. 180:1878-86.
11. Lindell, D. & Post, A. F. 2001. Ecological aspects of ntcA gene expression and its use as an indicator of the nitrogen status of marine Synechococcus. Appl. Environ. Microbiol. 67:3340-9.
12. Lindell, D. 2000. Assessing the Nitrogen Status of Marine Prokaryotic Phytoplankton Using Molecular Methods. Ph.D. thesis. Hebrew University of Jerusalem, Jerusalem, Israel, 126 pp.
13. Liu, H. B., Nolla, H. A. & Campbell, L. 1997. Prochlorococcus growth rate and contribution to primary production in the equatorial and subtropical North Pacific Ocean. Aquat. Microb. Ecol. 12: 39-47.
14. Liu, H. B., Landry, M. R., Vaulot, D. & Campbell, L. 1999. Prochlorococcus growth rates in the central equatorial Pacific: An application of the f(max) approach. J. Geophys. Res. Oceans 104:3391-9.
15. Luque, I., Flores, E. & Herrero, A. 1994. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 13:2862-9.
16. Marie, D., Partensky, F. & Vaulot, D. 1997. Enumeration and cell cycle analysis of marine picoplankton by flow cytometry using the nucleic stain SYBR green. Appl. Environ. Microbiol. 63:186-93.
17. Marini, A.-M., Vissers, S., Urrestarazu, A. & André, B. 1994. Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae. EMBO J. 13:3456-63.
18. Montesinos, M. L., Muro-Pastor, A. M., Herrero, A. & Flores, E. 1998. Ammonium/methylammonium permeases of a cyanobacterium. J. Biol. Chem. 273:31463-70.
19. Moore, L. R., Post, A. F., Rocap, G. & Chisholm, S. W. 2002. Utilization of different nitrogen sources by the marine cyanobacteria, Prochlorococcus and Synechococcus. Limnol. Oceanogr. 47:989-96.
20. Moyal, A. 2000, Control of the Assimilation of Nitrogen Compounds in the Marine Cyanobacterium Synechococcus sp. Strain WH 7803: Confirmation of Activity of the Genes ntcA and napA by Generation and Characterization of Mutants. M.Sc. thesis. Tel Aviv University, Tel Aviv, Israel, 90 pp.
21. Muro-Pastor, A. M., Herrero, A. & Flores, E. 2001a. Nitrogen-regulated group 2 sigma factor from Synechocystis sp. strain PCC 6803 involved in survival under nitrogen stress. J. Bacteriol. 183: 1090-5.
22. Muro-Pastor, M. I., Reyes, J. C. & Florencio, F. J. 2001b. Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem. 276:38320-8.
23. Partensky, F., Hess, W. R. & Vaulot, D. 1999. Prochlorococcus, a marine photosynthetic prokaryote of global significance. Microbiol. Mol. Biol. Rev. 63:106-27.
24. + uptake in roots of Arabidopsis thaliana: Evidence for regulation by root glutamine levels. Plant J. 19:143-52.
25. Rippka R, Coursin, T. Hess, W. R., Lichtlé, C., Scanlan, D. J., Palinska, K., Iteman, I., Partensky, F., Houmard, J. & Herdman, M. 2000. Prochlorococcus marinus Chisholm et al. 1992, subsp, nov. pastoris, strain PCC 9511, the first axenic chlorophyll a2/b2-containing cyanobacterium. Int. J. Syst. Evol. Microbiol. 50:1833-47.
26. Sauer, J., Margit, G. & Forchhammer, K. 1999. Nitrogen starvation in Synechococcus PCC 7942: Involvement of glutamine synthetase and NtcA in phycobiliprotein degradation and survival. Arch. Microbiol. 172:247-55.
27. Sauer, J., Dirmeier, U. & Forchhammer, K. 2000. The Synechococcus strain PCC 7942 glnN product (glutamine synthetase III) helps recovery from prolonged nitrogen chlorosis. J. Bacteriol. 182:5615-9.
28. Saunders, S. E. & Burke, J. F. 1990. Rapid isolation of miniprep DNA for double strand sequencing. Nucleic Acids Res. 18:4948.
29. 2-regulated promoters. Gene 90:43-9.
30. Steglich, C., Behrenfeld, M., Koblizek, M., Claustre, H., Penno, S., Prasil, O., Partensky, F. & Hess, W. R. 2001. Nitrogen deprivation strongly affects Photosystem II but not phycoerythrin level in the divinyl-chlorophyll b-containing cyanobacterium Prochlorococcus marinus. Biochim. Biophys. Acta 1503:341-9.
31. Tanigawa, R., Shirokane, M., Maeda, S., Omata, T., Tanaka, K., Takahashi, H. & Takahashi, H. 2002. Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. USA 99:4251-5.
32. Valladares, A, Montesinos, M. L., Herrero, A. & Flores, E. 2002. An ABC-type, high-affinity urea permease identified in cyanobacteria. Mol. Microbiol. 43:703-15.
33. Vazquez-Bermudez, M. F., Paz-Yepes, J., Herrero, A. & Flores, E. 2002. The NtcA-activated amt1 gene encodes a permease required for uptake of low concentrations of ammonium in the cyanobacterium Synechococcus sp. PCC 7942. Microbiology 148:861-9.
34. Vega-Palas, M. A., Madueno, F., Herrero, A. & Flores, E. 1990. Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 172:643-7.
35. 11 protein. J. Bacteriol. 176:108-14.