Protein-protein cross-linking in the use of the eukaryotic eGST-fusion system

Protein Expression and Purification

Volumn 26, Issue 3, 2002, Pages 462-466

  Papaioannou, Maria ^a   Ruppert, Thomas ^a   Dotzlaw, Helmut ^a   Dressel, Uwe ^a,b   Baniahmad, Aria ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTIFACTS; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; HEAT; MACROMOLECULAR SUBSTANCES; MERCAPTOETHANOL; MOLECULAR WEIGHT; PEPTIDE MAPPING; PROTEIN BINDING; PROTEINS; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EUKARYOTA;

EID: 0036910572     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00556-9     Document Type: Article

References (9)

1. J.D. Fondell, H. Ge, R.G. Roeder, Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex, Proc. Natl. Acad. Sci. USA 93 (1996) 8329-8333.
2. P. Alen, F. Claessens, G. Verhoeven, W. Rombauts, B. Peeters, The androgen receptor amino-terminal domain plays a key role in p160 coactivator-stimulated gene transcription, Mol. Cell Biol. 19 (1999) 6085-6097.
3. M. Ito, R.G. Roeder, The TRAP/SMCC/mediator complex and thyroid hormone receptor function, Trends Endocrinol. Metab. 12 (2001) 127-134.
4. R.Y. Tsai, R.R. Reed, Using a eukaryotic GST fusion vector for proteins difficult to express in E. coli, Biotechniques 23 (1997) 794-796, 798, 800.
5. L.J. Burke, A. Baniahmad, Co-repressors 2000, FASEB J. 14 (2000) 1876-1888.
6. U. Dressel, D. Thormeyer, B. Altincicek, A. Paululat, M. Eggert, S. Schneider, S.P. Tenbaum, R. Renkawitz, A. Baniahmad, Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily, Mol. Cell. Biol. 19 (1999) 3383-3394.
7. S. Cannon-Carlson, J. Tang, Modification of the Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis procedure to eliminate artifacts on reducing and nonreducing gels, Anal. Biochem. 246 (1997) 146-148.
8. D.N. Perkins, D.J. Pappin, D.M. Creasy, J.S. Cottrell, Probability-based protein identification by searching sequence databases using mass spectrometry data, Electrophoresis 20 (1999) 3551-3567.
9. A. Niedziela-Majka, G. Rymarczyk, M. Kochman, A. Ozyhar, GST-Induced dimerization of DNA-binding domains alters characteristics of their interaction with DNA, Protein Expr. Purif. 14 (1998) 208-220.