Immobilized, thermostable S- and F-forms of the extracellular invertase from Candida utilis can hydrolyse sucrose up to 100°C

Biotechnology Letters

Volumn 24, Issue 23, 2002, Pages 1993-1998

  Belcarz, Anna ^a   Ginalska, Grazyna ^a   Lobarzewski, Jerzy ^a  

^a MARIA CURIE SK ODOWSKA UNIVERSITY   (Poland)

Author keywords

D fructofuranosidase; Candida utilis; Immobilization; Invertase; Thermostability

Indexed keywords

CANDIDA UTILIS;

EID: 0036905377     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1021138101897     Document Type: Article

References (22)

1. Aksu Z, Kutsal T (1986) Lactic acid production from molasses utilizing Lactobacillus delbrueckii and invertase together. Biotechnol. Lett. 8: 157-160.
2. Belcarz A, Ginalska G, Lobarzewski J. Penel C (2002) The novel, non-glycosylated invertase form Candida utilis (conditions of biosynthesis, purification and properties). Biochim. Biophys. Acta 1594: 40-53.
3. Boze H, Moulin G, Galzy P (1994) Production of food and fodder yeast. Crit. Rev. Biotechnol. 12: 65-86.
4. Chavez FP, Rodriguez L, Diaz J, Delgado JM, Cremata JA (1997) Purification and characterization of an invertase from Candida utilis: Comparison with natural and recombinant yeast invertases. J. Biotechnol. 53: 67-74.
5. Chiang CJ, Lee WC (1997) Immobilization of β-fructofuranosidases from Aspergillus on methacrylamide-based polymeric beads for production of fructooligosaccharides. Biotechnol. Progr. 13: 577-582.
6. Ginalska G, Belcarz A, Lobarzewski J, Wolski T (1999) Application of immobilized invertase for continuous production of inverted sucrose. Biotechnologia 1: 276-237.
7. Huang CT, Lee WC (1994) Immobilization of invertase onto a copolymer of methacrylamide and N,N′-methlene-bis(acrylamide). Appl. Biochem. Biotechnol. 45/46: 663-672.
8. Husain S, Jafri F. Saleemuddin M (1996) Effects of chemical modification on the stability of invertase before and after immobilization. Enzyme Microb. Technol. 18: 275-280.
9. Iizuka M, Yamamoto T (1972) Studies on invertase of Candida utilis. Part I. Comparative study on isolation methods of the enzyme. Agr. Biol. Chem. Tokyo 36: 349-355.
10. Iizuka M, Tsuji Y, Yamamoto T (1974) Some physicochemical properties of invertase of Candida utilis. Agr. Biol. Chem. Tokyo 38: 213-215.
11. Kurokawa Y, Suzuki K, Tamai Y (1998) Adsorption and enzyme (β-galactosidase and a-chymotrypsin): Immobilization properties of gel fiber prepared by the gel formation of cellulose acetate and titanium iso-propoxide. Biotechnol. Bioeng. 59: 651-656.
12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. Lloyd JB, Whelan WJ (1969) An improved method for enzymic determination of glucose in the presence of maltose. Anal. Biochem. 30: 467-490.
14. Mansfield J, Forster M, Hoffmann T, Schellenberger A (1995) Coimmobilization of Yarrowia lipolytica cells and invertase in polyelectrolyte complex microcapsules. Enzyme Microb. Technol. 17: 11-17.
15. Monsan P, Combes D (1984) Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions. Biotechnol. Bioeng. 26: 347-351.
16. Naundorf A, Caussette M, Ajisaka K (1998) Characterization of the immobilized β-galactosidase C from Bacillus circulans and the production of β-(1-3)-linked disaccharides. Biosci. Biotech. Bioch. 62: 1313-1317.
17. Nikiel S (1996) Sugar Industry, 3rd Edn. Warszawa. Poland: WSP.
18. Schacterle GR, Pollack RL (1973) A simplified method for the quantitative assay of small amounts of protein in biologic material Anal. Biochem. 51: 654-655.
19. Selampinar F, Akbulut U, Ozden MY, Toppare L (1997) Immobilization of invertase in conducting polymer matrices. Biomaterials 18: 1163-1168.
20. Tull D, Gottschalk TE, Svendsen I, Kramhøft B, Phillipson BA, Bisgård-Frantzen H, Olsen O, Svensson B (2001) Extensive N-glycosylation reduces the thermal stability of a recombinant alkalophilic Bacillus α-amylase produced in Pichia pastoris. Protein Expres. Purif. 21: 13-23.
21. Weethall H (1976) Covalent coupling methods for inorganic support material. Method Enzymol. 44: 134-148.
22. Zaborsky OR, Ogletree J (1974) The immobilization of glucose oxidase via activation of its carbohydrate residues. Biochem. Biophys. Res. Co. 61: 210-216.