Complete disulfide bond assignment of a recombinant immunoglobulin G4 monoclonal antibody

Analytical Biochemistry

Volumn 311, Issue 1, 2002, Pages 1-9

  Zhang, Wei ^a,b   Marzilli, Lisa A ^a   Rouse, Jason C ^a   Czupryn, Marta J ^a  

^a PFIZER INC   (United States)

^b IMMUNOGEN INC   (United States)

Author keywords

Disulfide bond; Endoproteinase Lys C peptide map; Mass spectrometry; Monoclonal antibody; N terminal Edman sequencing; Reversed phase HPLC

Indexed keywords

COVALENT BONDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES;

DISULFIDE BONDS; DISULFIDE-CONTAINING PEPTIDES; GUANIDINE HYDROCHLORIDE; N-TERMINAL EDMAN SEQUENCING; QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETRY; RECOMBINANT MONOCLONAL ANTIBODY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REVERSED PHASE HPLC;

SULFUR COMPOUNDS;

AMINO ACID; CYSTEINE; DITHIOTHREITOL; GUANIDINE; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G4; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; LYSINE; MONOCLONAL ANTIBODY; N ETHYLMALEIMIDE; RECOMBINANT ANTIBODY; DISULFIDE; IMMUNOGLOBULIN G; METALLOPROTEINASE; PEPTIDE; PEPTIDYL LYS METALLOENDOPEPTIDASE; PEPTIDYL-LYS METALLOENDOPEPTIDASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CHO CELL; DIGESTION; DISULFIDE BOND; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; RECOMBINANT DNA TECHNOLOGY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ANIMAL; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; HAMSTER; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CYSTEINE; DISULFIDES; ETHYLMALEIMIDE; GUANIDINE; HUMANS; IMMUNOGLOBULIN G; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PEPTIDES; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 0036901746     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00394-9     Document Type: Article

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