Leaderless mRNAs bind 70S ribosomes more strongly than 30S ribosomal subunits in Escherichia coli

Journal of Bacteriology

Volumn 184, Issue 23, 2002, Pages 6730-6733

  O'Donnell, Sean M ^a   Janssen, Gary R ^a  

^a MIAMI UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INITIATION FACTOR 3; MESSENGER RNA;

ARTICLE; BACTERIAL GENETICS; BINDING AFFINITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; RIBOSOME SUBUNIT; RNA TRANSLATION; START CODON;

CODON, INITIATOR; ESCHERICHIA COLI; PROKARYOTIC INITIATION FACTORS; PROTEIN BIOSYNTHESIS; RIBOSOMES; RNA, MESSENGER;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036889040     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.23.6730-6733.2002     Document Type: Article

References (26)

1. Balakin, A. G., E. A. Skripkin, I. N. Shatsky, and A. A. Bogdanov. 1992. Unusual ribosome binding properties of mRNA encoding bacteriophage λ repressor. Nucleic Acids Res. 20:563-571.
2. Carter, A. P., W. M. Clemons, D. E. Brodersen, R. J. Morgan-Warren, T. Hartsch, B. T. Wimberly, and V. Ramakrishnan. 2001. Crystal structure of an initiation factor bound to the 30S ribosomal subunit. Science 291:498-501.
3. Christie, G. E., and R. Calendar. 1985. Bacteriophage P2 late promoters II. Comparison of the four late promoter sequences. J. Mol. Biol. 181:373-382.
4. Grill, S., C. O. Gualerzi, P. Londei, and U. Balsi. 2000. Selective stimulation of translation of leaderless mRNA by initiation factor 2: Evolutionary implications for translation. EMBO J. 19:1-10.
5. Grill, S., I. Moll, D. Hasenohrl, C. O. Gualerzi, and U. Blasi. 2001. Modulation of ribosomal recruitment to 5′-terminal start codons by translation initiation factors IF2 and IF3. FEBS Lett. 495:167-171.
6. Gualerzi, C. O., and C. L. Pon. 1990. Initiation of mRNA translation in prokaryotes. Biochemistry 29:5881-5889.
7. Hartz, D., D. S. McPheeters, R. Traut, and L. Gold. 1988. Extension inhibition analysis of translation initiation complexes. Methods Enzymol. 164:419-425.
8. Hui, A. J., and H. A. deBoer. 1987. Specialized ribosome system: Preferential translation of a single mRNA species by a subpopulation of mutated ribosomes in Escherichia coli. Proc. Natl. Acad. Sci. USA 84:4762-4766.
9. Jacob, W. F., M. Santer, and A. E. Dahlberg. 1987. A single base change in the Shine-Dalgarno region of the 16S rRNA of Escherichia coli affects translation of many proteins. Proc. Natl. Acad. Sci. USA 84:4757-4761.
10. Klock, G., and W. Hillen. 1986. Expression, purification, and operator binding of the transposon Tn1721-encoded Tet repressor. J. Mol. Biol. 189:633-641.
11. LaTeana, A., C. O. Gualerzi, and A. E. Dahlberg. 2001. Initiation factor IF2 binds to the α-sarcin loop and helix 89 of Escherichia coli 23S ribosomal RNA. RNA 7:1173-1179.
12. Martin-Farmer, J., and G. R. Janssen. 1999. A downstream CA repeat sequence increases translation from leadered and unleadered mRNA in Escherichia coli. Mol. Microbiol. 31:1025-1038.
13. McCutcheon, J. P., R. K. Agrawal, S. M. Phillios, R. A. Grassucci, S. E. Gerchman, W. M. Clemons, V. Ramakrishnan, and J. Frank. 1999. Location of translation initiation factor IF3 on the small ribosomal subunit. Proc. Natl. Acad. Sci. USA 96:4301-4306.
14. Moazed, D., R. R. Samaha, C. Gualerzi, and H. F. Noller. 1995. Specific protection of 16S rRNA by translation initiation factors. J. Mol. Biol. 248:207-210.
15. Moll, I., A. Resch, and U. Blasi. 1998. Discrimination of 5′-terminal start codons by translation initiation factor 3 is mediated by ribosomal protein S1. FEBS Lett. 436:213-217.
16. O'Donnell, S. M., and G. R. Janssen. 2001. The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5′ untranslated leader. J. Bacteriol. 183:1277-1283.
17. Petrelli, D., A. LaTeana, C. Garofalo, R. Spurio, C. L. Pon, and C. O. Gualerzi. 2001. Translation initiation factor IF3: Two domains, five functions, one mechanism? EMBO J. 20:4560-4569.
18. Pon, C. L., M. Paci, R. T. Pawlik, and C. O. Gualerzi. 1985. Structure-function relationship in Escherichia coli initiation factors. J. Biol. Chem. 260:8918-8924.
19. Ramesh, V., S. Gite, and U. L. RajBhandary. 1998. Functional interaction of an arginine conserved in the sixteen amino acid insertion module of Escherichia coli methionyl-tRNA formyltransferase with determinants for formylation in the initiator tRNA. Biochemistry 37:15925-15932.
20. Ringquist, S., S. Shinedling, D. Barrick, L. Green, J. Binkley, G. D. Stormo, and L. Gold. 1992. Translation initiation in Escherichia coli: Sequences within the ribosome-binding site. Mol. Microbiol. 6:1219-1229.
21. Shine, J., and L. Dalgarno. 1974. The 3′ terminal of Escherichia coli 16S ribosomal RNA: Complementarity to non-sense triplets and ribosomal binding sites. Proc. Natl. Acad. Sci. USA 71:1342-1346.
22. Soffientini, A., R. Lorenzetti, L. Gastaldo, J. H. Parlett, R. Spurio, A. LaTeana, and K. Islam. 1994. Purification procedure for bacterial translational initiation factors IF2 and IF3. Protein Expr. Purif. 5:118-124.
23. Spedding, G. 1990. Isolation and analysis of ribosomes from prokaryotes, eukaryotes, and organelles, p. 1-27. In G. Spedding (ed.), Ribosomes and Protein Synthesis: A Practical Approach. Oxford University Press, Oxford, United Kingdom.
24. Tedin, K., I. Moll, S. Grill, A. Resch, A. Graschopf, C. O. Gualerzi, and U. Blasi. 1999. Translation initiation factor 3 antagonizes authentic start codon selection on leaderless mRNAs. Mol. Microbiol. 31:67-77.
25. Van Etten, W. J., and G. R. Janssen. 1998. An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli. Mol. Microbiol. 27:987-1001.
26. Wu, C.-J., and G. R. Janssen. 1996. Translation of vph mRNA in Streptomyces lividans and Escherichia coli after removal of the 5′ untranslated leader. Mol. Microbiol. 22:339-355.