Construction of a Tcl-like transposon Sleeping Beauty-based gene transfer plasmid vector for generation of stable transgenic mammalian cell clones

Analytical Biochemistry

Volumn 310, Issue 1, 2002, Pages 15-26

  Harris, Joseph W ^a   Strong, Donna D ^a   Amoui, Mehran ^a   Baylink, David J ^a   Lau, K H William ^a  

^a LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Gene transfer; Plasmid; Sleeping Beauty; Stable transfection; Transposon

Indexed keywords

CLONE CELLS; CYTOLOGY; DNA; GENE TRANSFER; MAMMALS; PLANTS (BOTANY); SLEEP RESEARCH; SULFUR COMPOUNDS; VECTORS;

GENE TRANSFER VECTORS; PLASMID; POLYADENYLATION SIGNALS; RED FLUORESCENT PROTEINS; SELECTION PRESSURES; SLEEPING BEAUTY; STABLE TRANSFECTION; TRANSPOSON;

GENES;

GENE PRODUCT; PLASMID VECTOR; PROTEIN TCL; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG; VIRUS VECTOR; PHOTOPROTEIN; TRANSPOSASE;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CONTROLLED STUDY; CYTOMEGALOVIRUS; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INVERTED REPEAT; MOLECULAR CLONING; MOLECULAR STABILITY; NONHUMAN; OSTEOSARCOMA CELL; POLYADENYLATION; PRIORITY JOURNAL; PROMOTER REGION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; TRANSPOSON; CELL CLONE; CELL CULTURE; CHROMOSOME INVERSION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; METHODOLOGY; NUCLEOTIDE REPEAT; OSTEOSARCOMA; PLASMID; ULTRASTRUCTURE;

CUCUMBER MOSAIC VIRUS; MAMMALIA; RICE STRIPE VIRUS;

CLONE CELLS; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; FLOW CYTOMETRY; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; INVERSION, CHROMOSOME; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MUTAGENESIS, INSERTIONAL; OSTEOSARCOMA; PLASMIDS; REPETITIVE SEQUENCES, NUCLEIC ACID; TRANSFECTION; TRANSFORMATION, GENETIC; TRANSPOSASES; TUMOR CELLS, CULTURED;

EID: 0036854185     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00316-0     Document Type: Article

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