Conformational dependence and conservation of an immunodominant epitope within the Babesia equi erythrocyte-stage surface protein equi merozoite antigen 1

Clinical and Diagnostic Laboratory Immunology

Volumn 9, Issue 6, 2002, Pages 1301-1306

  Cunha, Cristina W ^a   Kappmeyer, Lowell S ^a   McGuire, Travis C ^a   Dellagostin, Odir A ^a   Knowles, Donald P ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; EQUI MEROZOITE ANTIGEN 1; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY; PARASITE ANTIBODY; PARASITE ANTIGEN; RECOMBINANT ANTIGEN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; ANTIGEN RECOGNITION; ARTICLE; BABESIA EQUI; CARBOXY TERMINAL SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTE; ESCHERICHIA COLI; GENETIC CONSERVATION; HORSE; HORSE DISEASE; IMMUNOREACTIVITY; LIFE CYCLE; MEROZOITE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN EXPRESSION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, PROTOZOAN; BABESIA; CLONING, MOLECULAR; ERYTHROCYTES; HORSES; IMMUNODOMINANT EPITOPES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS;

EID: 0036843576     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.9.6.1301-1306.2002     Document Type: Article

References (24)

1. Callow, L. L., W. McGregor, B. J. Rodwell, R. J. Rogers, G. C. Fraser, D. F. Mahoney, and G. M. Robertson. 1979. Evaluation of an indirect fluorescent antibody test to diagnose Babesia equi infection in horses. Aust. Vet. J. 55:555-559.
2. Frerichs, W. M., A. A. Holbrook, and A. J. Johnson. 1969. Equine piroplasmosis: Complement-fixation titers of horses infected with Babesia caballi. Am. J. Vet. Res. 30:697-702.
3. Frerichs, W. M., A. A. Holbrook, and A. J. Johnson. 1969. Equine piroplasmosis: Production of antigens for the complement-fixation test. Am. J. Vet. Res. 30:1337-1341.
4. Friedhoff, K. T., and C. Soule. 1996. An account on equine babesioses. Rev. Sci. Tech. 15:1191-1201.
5. Friedhoff, K. T., A. M. Tenter, and I. Muller. 1990. Haemoparasites of equines: Impact on international trade of horses. Rev. Sci. Tech. 9:1187-1194.
6. Gubbels, M. J., F. Katzer, G. Hide, F. Jongejan, and B. R. Shiels. 2000. Generation of a mosaic pattern of diversity in the major merozoite-piroplasm surface antigen of Theileria annulata. Mol. Biochem. Parasitol. 110:23-32.
7. Hanafusa, Y., T. Sudo, Y. Sako, T. Kanemaru, M. Kamada, E. Zweygarth, C. Sugimoto, and M. Onuma. 1998. Identification of B cell epitopes of a 30 kDa Babesia equi merozoite surface protein. J. Vet. Med. Sci. 60:563-567.
8. Horiuchi, M., N. Yamazaki, T. Ikeda, N. Ishiguro, and M. Shinagawa. 1995. A cellular form of prion protein (PrPC) exists in many non-neuronal tissues of sheep. J. Gen. Virol. 76(Pt. 10):2583-2587.
9. Kappmeyer, L. S., L. E. Perryman, and D. P. Knowles, Jr. 1993. A Babesia equi gene encodes a surface protein with homology to Theileria species. Mol. Biochem. Parasitol. 62:121-124.
10. Katz, J., R. Dewald, and J. Nicholson. 2000. Procedurally similar competitive immunoassay systems for the serodiagnosis of Babesia equi, Babesia caballi, Trypanosoma equiperdum, and Burkholderia mallei infection in horses. J. Vet. Diagn. Investig. 12:46-50.
11. Katzer, F., S. McKellar, E. Kirvar, and B. Shiels. 1998. Phylogenetic analysis of Theileria and Babesia equi in relation to the establishment of parasite populations within novel host species and the development of diagnostic tests. Mol. Biochem. Parasitol. 95:33-44.
12. Knowles, D. P., L. S. Kappmeyer, and L. E. Perryman. 1997. Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species. Mol. Biochem. Parasitol. 90:69-79.
13. Knowles, D. P., Jr., L. S. Kappmeyer, D. Stiller, S. G. Hennager, and L. E. Perryman. 1992. Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi. J. Clin. Microbiol. 30:3122-3126.
14. Knowles, D. P., Jr., L. E. Perryman, W. L. Goff, C. D. Miller, R. D. Harrington, and J. R. Gorham. 1991. A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites. Infect. Immun. 59:2412-2417.
15. Knowles, D. P., Jr., L. E. Perryman, L. S. Kappmeyer, and S. G. Hennager. 1991. Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. J. Clin. Microbiol. 29:2056-2058.
16. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
17. Laveran, A. 1901. Contribution á l'étude de Piroplasma equi. C. R. Soc. Biol. 53:385-388.
18. Rhalem, A., H. Sahibi, S. Lasri, W. C. Johnson, L. S. Kappmeyer, A. Hamidouch, D. P. Knowles, and W. L. Goff. 2001. Validation of a competitive enzyme-linked immunosorbent assay for diagnosing Babesia equi infections of Moroccan origin and its use in determining the seroprevalence of B. equi in Morocco. J. Vet. Diagn. Investig. 13:249-251.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory, N.Y.
20. Schein, E. 1988. Equine babesiosis, p. 197-208. In M. Ristic (ed.), Babesiosis of Domestic Animals and Man. CRC Press, Inc., Boca Raton, Fla.
21. Shkap, V., I. Cohen, B. Leibovitz, Savitsky, E. Pipano, G. Avni, S. Shofer, U. Giger, L. Kappmeyer, and D. Knowles. 1998. Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays. Vet. Parasitol. 76:251-259.
22. Tanaka, T., X. Xuan, H. Ikadai, I. Igarashi, H. Nagasawa, K. Fujisaki, T. Mikami, and N. Suzuki. 1999. Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA. Int. J. Parasitol. 29:1803-1808.
23. Xuan, X., I. Igarashi, T. Tanaka, S. Fukumoto, H. Nagasawa, K. Fujisaki, and T. Mikami. 2001. Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1. Clin. Diagn. Lab. Immunol. 8:645-646.
24. Xuan, X., A. Larsen, H. Ikadai, T. Tanaka, I. Igarashi, H. Nagasawa, K. Fujisaki, Y. Toyoda, N. Suzuki, and T. Mikami. 2001. Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. J. Clin. Microbiol. 39:705-709.