Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA

International Journal for Parasitology

Volumn 29, Issue 11, 1999, Pages 1803-1808

  Tanaka, Tetsuya ^a   Xuan, Xuenan ^a   Ikadai, Hiromi ^a   Igarashi, Ikuo ^a   Nagasawa, Hideyuki ^a   Fujisaki, Kozo ^a   Mikami, Takeshi ^a   Suzuki, Naoyoshi ^a  

^a OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

Author keywords

Babesia equi; Baculovirus; ELISA; EMA 2

Indexed keywords

PARASITE ANTIGEN; TUNICAMYCIN;

AMINO ACID SEQUENCE; ANIMAL MODEL; ANTIBODY RESPONSE; ANTIGEN EXPRESSION; BABESIA; BABESIA EQUI; BACULOVIRUS; CELL CULTURE; CLONING VECTOR; CONTROLLED STUDY; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENTAL INFECTION; GENE INSERTION; GLYCOSYLATION; HORSE; INSECT; MEROZOITE; NONHUMAN; SHORT SURVEY; VIRUS RECOMBINANT;

BABESIA EQUI; EQUIDAE; INSECTA; MURINAE; UNIDENTIFIED BACULOVIRUS;

EID: 0032720546     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0020-7519(99)00146-0     Document Type: Article

References (14)

1. Schein E. Equine babesiosis. In: Ristic M, editor. Babesiosis of domestic animals and man. Boca Raton, FL: CRC Press, 1988; 197-208.
2. Knowles R.C. Equine babesiosis: epidemiology, control and chemotherapy. Equine Vet Sci. 8:1988;61-64.
3. Brüning A. Equine piroplasmosis: an update on diagnosis, treatment and prevention. Br Vet J. 152:1996;139-151.
4. Holbrook A.A. Biology of equine piroplasmosis. Am J Vet Med Assoc. 155:1969;453-454.
5. Kappmeyer L.S., Perryman L.E., Knowles D.P. Jr. A Babesia equi gene encodes a surface protein with homology to Theileria species. Mol Biochem Parasitol. 62:1993;121-124.
6. Knowles D.P. Jr., Kappmeyer L.S., Stiller D., Hennager S.G., Perryman L.E. Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi. J Clin Microbiol. 30:1992;3122-3126.
7. Knowles D.P. Jr., Kappmeyer L.S., Perryman L.E. Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species. Mol Biochem Parasitol. 90:1997;69-79.
8. Avarzed A., Igarashi I., De Waal D.T.et al. Monoclonal antibody against Babesia equi: characterization and potential application of antigen for serodiagnosis. J Clin Microbiol. 36:1998;1835-1839.
9. Avarzed A., Igarashi I., Kanemaru T.et al. Improved in vitro cultivation of Babesia caballi. J Vet Med Sci. 59:1998;479-481.
10. Xuan X., Maeda K., Mikami T., Otsuka H. Characterization of canine herpes virus glycoprotein C expressed by a recombinant baculovirus in insect cells. Virus Res. 46:1996;57-64.
11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
12. Ei-Ghaysh A., Sundquist B., Christensson D.A., Hilali M., Nassar A.M. Observations on the use of ELISA for detection of Babesia bigemina specific antibodies. Vet Parasitol. 62:1996;51-61.
13. Tenter A.M., Friedhoff K.T. Serodiagnosis of experimental and natural Babesia equi and B. caballi infections. Vet Parasitol. 20:1986;49-61.
14. Weiland G, Reiter I. Methods for the measurement of the serological response to Babesia. In: Ristic M, editor. Babesiosis of domestic animals and man. Boca Raton, FL: CRC Press, 1988; 143-162.