Sir2p and Sas2p opposingly regulate acetylation of yeast histone H4 lysine16 and spreading of heterochromatin

Nature Genetics

Volumn 32, Issue 3, 2002, Pages 378-383

  Suka, Noriyuki ^a   Luo, Kunheng ^a   Grunstein, Michael ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; HISTONE ACETYLTRANSFERASE; LYSINE; PROTEIN SAS2P; SILENT INFORMATION REGULATOR PROTEIN 2; UNCLASSIFIED DRUG;

ACETYLATION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; EUCHROMATIN; GENE DISRUPTION; HETEROCHROMATIN; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REGULATORY MECHANISM;

ACETYLATION; ACETYLTRANSFERASES; ARGININE; CHROMATIN; HETEROCHROMATIN; HISTONE ACETYLTRANSFERASES; HISTONE DEACETYLASES; HISTONES; LYSINE; MUTATION; PRECIPITIN TESTS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE PROTEINS; SILENT INFORMATION REGULATOR PROTEINS, SACCHAROMYCES CEREVISIAE; SIRTUINS; TELOMERE;

PROKARYOTA;

EID: 0036842129     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng1017     Document Type: Article

References (33)

1. Braunstein, M., Rose, A.B., Holmes, S.G., Allis, C.D. & Broach, J.R. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation. Genes Dev. 7, 592-604 (1993).
2. Suka, N., Suka, Y., Carmen, A.A., Wu, J. & Grunstein, M. Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin. Mol. Cell 8, 473-479 (2001).
3. Moretti, P., Freeman, K., Coodly, L. & Shore, D. Evidence that a complex of Sir proteins interacts with the silencer and telomere-binding protein RAP1. Genes Dev. 8, 2257-2269 (1994).
4. Grunstein, M. Molecular model for telomeric heterochromatin in yeast. Curr. Opin. Cell Biol. 9, 383-387 (1997).
5. Johnson, L.M., Kayne, RS., Kahn, E.S. & Grunstein, M. Genetic evidence for an interaction between Sir3p and histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA 87, 6286-6290 (1990).
6. Megee, RC., Morgan, B.A., Mittman, B.A. & Smith, M.M. Genetic analysis of histone H4: essential role of lysines subject to reversible acetylation. Science 247, 841-845 (1990).
7. Carmen, A.A., Milne, L. & Grunstein, M. Acetylation on the yeast histone H4 N terminus regulates its binding to heterochromatin protein Sir3p. J. Biol. Chem. 277, 4778-4781 (2002).
8. Lachner, M., O'Carroll, D., Rea, S., Mechtler, K. & Jenuwein, T. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Nature 410, 116-120 (2001).
9. Bannister, A.J. et al. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain. Nature 410, 120-124 (2001).
10. Litt, M.D., Simpson, M., Gaszner, M., Allis, C.D. & Felsenfeld, G. Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus. Science 293, 2453-2455 (2001).
11. Nakayama, J., Rice, J.C., Strahl, B.D., Allis, C.D. & Grewal, S.I. Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly. Science 292, 110-113 (2001).
12. Strahl, B.D., Ohba, R., Cook, R.G. & Allis, C.D. Methylation of histone H3 at lysine 4 is highly conserved and correlates with transcriptionally active nuclei in Tetrahymena. Proc. Natl Acad. Sci. USA 96, 14967-14972 (1999).
13. Strahl, B.D. et al. Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol. Cell. Biol. 22, 1298-1306 (2002).
14. Donze, D. & Kamakaka, R.T. RNA polymerase III and RNA polymerase II promoter complexes are heterochromatin barriers in Saccharomyces cerevisiae. EMBO J. 20, 520-531 (2001).
15. Clarke, D.J., O'Neill, L.P. & Turner, B.M. Selective use of H4 acetylation sites in the yeast Saccharomyces cerevisiae. Biochem J. 294, 557-561 (1993).
16. Grunstein, M. Yeast heterochromatin: Regulation of its assembly and inheritance by histones. Cell 93, 325-328 (1998).
17. Reifsnyder, C., Lowell, J., Clarke, A. & Pillus, L. Yeast SAS silencing genes and human genes associated with AML and HIV-1 Tat interactions are homologous with acetyltransferases. Nat. Genet. 14, 42-49 (1996).
18. Akhtar, A. & Becker, P.B. Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila. Mol. Cell 5, 367-375 (2000).
19. Smith, E.R. et al. The Drosophila MSL complex acetylates histone H4 at lysine 16, a chromatin modification linked to dosage compensation. Mol. Cell. Biol. 20, 312-318 (2000).
20. Imai, S., Armstrong, C.M., Kaeberlein, M. & Guarente, L. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 403, 795-800 (2000).
21. Clarke, A.S., Lowell, J.E., Jacobson, S.J. & Pillus, L. Esa1p is an essential histone acetyltransferase required for cell cycle progression. Mol. Cell. Biol. 19, 2515-2526 (1999).
22. Renauld, H. et al. Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by Sir3p dosage. Genes Dev. 7, 1133-1345 (1993).
23. Hecht, A., Strahl-Bolsinger, S. & Grunstein, M. Spreading of transcriptional repressor Sir3p from telomeric heterochromatin. Nature 383, 92-96 (1996).
24. Strahl-Bolsinger, S., Hecht, A., Luo, K. & Grunstein, M. Sir2 and Sir4 interactions differ in core and extended telomeric heterochromatin in yeast. Genes Dev. 11, 83-93 (1997).
25. Johnson, L.M., Fisher-Adams, G. & Grunstein, M. Identification of a non-basic domain in the histone H4 N-terminus required for repression of the yeast silent mating loci. EMBO J. 11, 2201-2209 (1992).
26. Hecht, A., Laroche, T., Strahl-Bolsinger, S., Gasser, S.M. & Grunstein, M. Histone H3 and H4 N-termini interact with Sir3p and Sir4p proteins: a molecular model for the formation of heterochromatin in yeast. Cell 80, 583-592 (1995).
27. Kimura, A., Umehara, T. & Horikoshi, M. Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing. Nat. Genet. 32 (2002); advance online publication, 15 October 2002 (doi:10.10138/993).
28. Fourel, G., Revardel, E., Koering, C.E. & Gilson, E. Cohabitation of insulators and silencing elements in yeast subtelomeric regions. EMBO J. 18, 2522-2537 (1999).
29. Robyr, D. etal. Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases. Cell 109, 437-446 (2002).
30. Gu, W., Wei, X., Pannuti, A. & Lucchesi, J.C. Targeting the chromatin-remodeling MSL complex of Drosophila to its sites of action on the X chromosome requires both acetyl transferase and ATPase activities. EMBO J. 19, 5202-5211 (2000).
31. Wach, A. et al. PCR-based gene targeting in Saccharomyces cerevisiae. Method in Microbiology Vol. 26 (eds Brown, P.D.P. & Tuite, M.F.) 67-81 (Academic, London, 1998).
32. Vogelauer, M., Wu, J., Suka, N. & Grunstein, M. Global histone acetylation and deacetylation in yeast. Nature 408, 495-498 (2000).
33. Brown, A.J.P., Furness, L.M. & Bailey, D. Transcript analysis. Method in Microbiology Vol. 26 (eds Brown, P.D.P. & Tuite, M.F.) 119-139 (Academic, London, 1998).